Construct: ORF ccsbBroadEn_00565

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011115.1_s300c1, BRDN0000395941
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FH (2271)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2271 FH fumarate hydratase NM_000143.3 100% 100%
2 human 2271 FH fumarate hydratase XM_011544132.2 85% 85% 0_1ins228
3 mouse 14194 Fh1 fumarate hydratase 1 NM_010209.2 87% 92.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1596
ORF length:
1530
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTA CCGAGCACTT CGGCTCCTCG CGCGCTCGCG TCCCCTCGTG CGGGCTCCAG
121CCGCAGCCTT AGCTTCGGCT CCCGGCTTGG GTGGCGCGGC CGTGCCCTCG TTTTGGCCTC
181CGAACGCGGC TCGAATGGCA AGCCAAAATT CCTTCCGGAT AGAATATGAT ACCTTTGGTG
241AACTAAAGGT GCCAAATGAT AAGTATTATG GCGCCCAGAC CGTGAGATCT ACGATGAACT
301TTAAGATTGG AGGTGTGACA GAACGCATGC CAACCCCAGT TATTAAAGCT TTTGGCATCT
361TGAAGCGAGC GGCCGCTGAA GTAAACCAGG ATTATGGTCT TGATCCAAAG ATTGCTAATG
421CAATAATGAA GGCAGCAGAT GAGGTAGCTG AAGGTAAATT AAATGATCAT TTTCCTCTCG
481TGGTATGGCA GACTGGATCA GGAACTCAGA CAAATATGAA TGTAAATGAA GTCATTAGCA
541ATAGAGCAAT TGAAATGTTA GGAGGTGAAC TTGGCAGCAA GATACCTGTG CATCCCAACG
601ATCATGTTAA TAAAAGCCAG AGCTCAAATG ATACTTTTCC CACAGCAATG CACATTGCTG
661CTGCAATAGA AGTTCATGAA GTACTGTTAC CAGGACTACA GAAGTTACAT GATGCTCTTG
721ATGCAAAATC CAAAGAGTTT GCACAGATCA TCAAGATTGG ACGTACTCAT ACTCAGGATG
781CTGTTCCACT TACTCTTGGG CAGGAATTTA GTGGTTATGT TCAACAAGTA AAATATGCAA
841TGACAAGAAT AAAAGCTGCC ATGCCAAGAA TCTATGAGCT CGCAGCTGGA GGCACTGCTG
901TTGGTACAGG TTTAAATACT AGAATTGGCT TTGCAGAAAA GGTTGCTGCA AAAGTGGCTG
961CACTTACAGG CTTGCCTTTT GTCACTGCTC CGAATAAATT TGAAGCTCTG GCTGCTCATG
1021ACGCTCTGGT TGAGCTCAGT GGAGCCATGA ACACTACTGC CTGCAGTCTG ATGAAGATAG
1081CAAATGATAT TCGATTTTTG GGTTCTGGTC CTCGGTCAGG TCTGGGAGAA TTGATCTTGC
1141CTGAAAATGA ACCAGGAAGC AGTATCATGC CAGGCAAGGT GAACCCTACT CAGTGTGAAG
1201CAATGACCAT GGTTGCAGCC CAAGTCATGG GGAACCATGT TGCTGTCACT GTCGGAGGCA
1261GCAATGGACA TTTTGAGTTG AATGTTTTCA AGCCAATGAT GATTAAAAAT GTGTTACACT
1321CAGCCAGGCT GCTGGGGGAT GCTTCAGTTT CCTTTACAGA AAACTGCGTG GTGGGAATCC
1381AGGCCAATAC AGAAAGGATC AACAAGCTGA TGAATGAGTC TCTAATGTTG GTGACAGCTC
1441TCAATCCTCA TATAGGGTAT GACAAGGCAG CAAAGATTGC TAAGACAGCA CACAAAAATG
1501GATCAACCTT AAAGGAAACT GCTATCGAAC TTGGCTATCT CACAGCAGAG CAGTTTGACG
1561AATGGGTAAA ACCTAAGGAC ATGCTGGGTC CAAAGTGCCC AACTTTCTTG TACAAAGTtg
1621gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)

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