Construct: ORF ccsbBroadEn_00752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012857.1_s300c1, BRDN0000397823
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGB3 (3149)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3149 | HMGB3 | high mobility group box 3 | NM_001301228.1 | 100% | 100% | |
| 2 | human | 3149 | HMGB3 | high mobility group box 3 | NM_001301229.2 | 100% | 100% | |
| 3 | human | 3149 | HMGB3 | high mobility group box 3 | NM_005342.4 | 100% | 100% | |
| 4 | human | 3149 | HMGB3 | high mobility group box 3 | XM_024452369.1 | 100% | 100% | |
| 5 | human | 3149 | HMGB3 | high mobility group box 3 | NM_001301231.2 | 90.9% | 90.9% | 1_60del |
| 6 | human | 128872 | HMGB3P1 | high mobility group box 3 p... | NR_002165.1 | 57.9% | (many diffs) | |
| 7 | mouse | 15354 | Hmgb3 | high mobility group box 3 | NM_001293623.1 | 91.6% | 97.5% | (many diffs) |
| 8 | mouse | 15354 | Hmgb3 | high mobility group box 3 | NM_001293624.1 | 91.6% | 97.5% | (many diffs) |
| 9 | mouse | 15354 | Hmgb3 | high mobility group box 3 | NM_001293625.1 | 91.6% | 97.5% | (many diffs) |
| 10 | mouse | 15354 | Hmgb3 | high mobility group box 3 | NM_008253.4 | 91.6% | 97.5% | (many diffs) |
| 11 | mouse | 15354 | Hmgb3 | high mobility group box 3 | XM_006527842.3 | 91.6% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 666
- ORF length:
- 600
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TAAAGGTGAC CCCAAGAAAC CAAAGGGCAA GATGTCCGCT TATGCCTTCT 121 TTGTGCAGAC ATGCAGAGAA GAACATAAGA AGAAAAACCC AGAGGTCCCT GTCAATTTTG 181 CGGAATTTTC CAAGAAGTGC TCTGAGAGGT GGAAGACGAT GTCCGGGAAA GAGAAATCTA 241 AATTTGATGA AATGGCAAAG GCAGATAAAG TGCGCTATGA TCGGGAAATG AAGGATTATG 301 GACCAGCTAA GGGAGGCAAG AAGAAGAAGG ATCCTAATGC TCCCAAAAGG CCACCGTCTG 361 GATTCTTCCT GTTCTGTTCA GAATTCCGCC CCAAGATCAA ATCCACAAAC CCCGGCATCT 421 CTATTGGAGA CGTGGCAAAA AAGCTGGGTG AGATGTGGAA TAATTTAAAT GACAGTGAAA 481 AGCAGCCTTA CATCACTAAG GCGGCAAAGC TGAAGGAGAA GTATGAGAAG GATGTTGCTG 541 ACTATAAGTC GAAAGGAAAG TTTGATGGTG CAAAGGGTCC TGCTAAAGTT GCCCGGAAAA 601 AGGTGGAAGA GGAAGATGAA GAAGAGGAGG AGGAAGAAGA GGAGGAGGAG GAGGAGGAGG 661 ATGAATACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 721 gttgcaacga ac