Construct: ORF ccsbBroadEn_00789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000343.1_s300c1, BRDN0000389700
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSBP1 (3281)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3281 | HSBP1 | heat shock factor binding p... | NM_001537.4 | 100% | 100% | |
| 2 | mouse | 68196 | Hsbp1 | heat shock factor binding p... | NM_024219.1 | 85% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 294
- ORF length:
- 228
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CGAGACTGAC CCCAAGACCG TGCAGGACCT CACCTCGGTG GTGCAGACAC 121 TCCTGCAGCA GATGCAAGAT AAATTTCAGA CCATGTCTGA CCAGATCATT GGGAGAATTG 181 ATGATATGAG TAGTCGCATT GATGATCTGG AAAAGAATAT CGCGGACCTC ATGACACAGG 241 CTGGGGTGGA AGAACTGGAA AGTGAAAACA AGATACCTGC CACGCAAAAG AGTTGCCCAA 301 CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac