Construct: ORF ccsbBroadEn_00826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012619.1_s300c1, BRDN0000455503
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA7 (3444)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 100% | 100% | |
| 2 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 96.8% | 93.6% | (many diffs) |
| 3 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 95.9% | 92% | (many diffs) |
| 4 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 95.7% | 92% | (many diffs) |
| 5 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 94% | 87.8% | (many diffs) |
| 6 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 93.1% | 85.7% | (many diffs) |
| 7 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 90.1% | 80.9% | (many diffs) |
| 8 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 88.3% | 79.8% | (many diffs) |
| 9 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 87.8% | 77.7% | (many diffs) |
| 10 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 87.4% | 77.7% | (many diffs) |
| 11 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 87.3% | 77.7% | (many diffs) |
| 12 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 86.9% | 76.7% | (many diffs) |
| 13 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 86.3% | 75.7% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCCCGGTCC TTTTCTTTAC TGATGGTCGT GCTGGTACTC AGCTACAAAT 121 CCATCTGCTC TCTGGGCTGT GATCTGCCTC AGACCCACAG CCTGCGTAAT AGGAGGGCCT 181 TGATACTCCT GGCACAAATG GGAAGAATCT CTCCTTTCTC CTGCTTGAAG GACAGACATG 241 AATTCAGATT CCCAGAGGAG GAGTTTGATG GCCACCAGTT CCAGAAGACT CAAGCCATCT 301 CTGTCCTCCA TGAGATGATC CAGCAGACCT TCAATCTCTT CAGCACAGAG GACTCATCTG 361 CTGCTTGGGA ACAGAGCCTC CTAGAAAAAT TTTCCACTGA ACTTTACCAG CAACTGAATG 421 ACCTGGAAGC ATGTGTGATA CAGGAGGTTG GGGTGGAAGA GACTCCCCTG ATGAATGAGG 481 ACTTCATCCT GGCTGTGAGG AAATACTTCC AAAGAATCAC TCTTTATCTA ATGGAGAAGA 541 AATACAGCCC TTGTGCCTGG GAGGTTGTCA GAGCAGAAAT CATGAGATCC TTCTCTTTTT 601 CAACAAACTT GAAAAAAGGA TTAAGGAGGA AGGATTTGCC AACTTTCTTG TACAAAGTTG 661 Gcattataag aaagcattgc ttatcaattt gttgcaacga ac