Construct: ORF ccsbBroadEn_01307

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006282.1_s300c1, BRDN0000392172
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMA1 (5682)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5682 PSMA1 proteasome 20S subunit alpha 1 NM_002786.3 100% 100%
2 human 5682 PSMA1 proteasome 20S subunit alpha 1 NM_148976.2 97.6% 97.3% 1_18del;20A>T
3 human 5682 PSMA1 proteasome 20S subunit alpha 1 NM_001143937.1 47.2% 44.8% (many diffs)
4 mouse 26440 Psma1 proteasome (prosome, macrop... NM_011965.2 92% 98% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
855
ORF length:
789
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTT TCGAAATCAG TATGACAATG ATGTCACTGT TTGGAGCCCC CAGGGCAGGA
121TTCATCAAAT TGAATATGCA ATGGAAGCTG TTAAACAAGG TTCAGCCACA GTTGGTCTGA
181AATCAAAAAC TCATGCAGTT TTGGTTGCAT TGAAAAGGGC GCAATCAGAG CTTGCAGCTC
241ATCAGAAAAA AATTCTCCAT GTTGACAACC ATATTGGTAT CTCAATTGCG GGGCTTACTG
301CTGATGCTAG ACTGTTATGT AATTTTATGC GTCAGGAGTG TTTGGATTCC AGATTTGTAT
361TCGATAGACC ACTGCCTGTG TCTCGTCTTG TATCTCTAAT TGGAAGCAAG ACCCAGATAC
421CAACACAACG ATATGGCCGG AGACCATATG GTGTTGGTCT CCTTATTGCT GGTTATGATG
481ATATGGGCCC TCACATTTTC CAAACCTGTC CATCTGCTAA CTATTTTGAC TGCAGAGCCA
541TGTCCATTGG AGCCCGTTCC CAATCAGCTC GTACTTACTT GGAGAGACAT ATGTCTGAAT
601TTATGGAGTG TAATTTAAAT GAACTAGTTA AACATGGTCT GCGTGCCTTA AGAGAGACGC
661TTCCTGCAGA ACAGGACCTG ACTACAAAGA ATGTTTCCAT TGGAATTGTT GGTAAAGACT
721TGGAGTTTAC AATCTATGAT GATGATGATG TGTCTCCATT CCTGGAAGGT CTTGAAGAAA
781GACCACAGAG AAAGGCACAG CCTGCTCAAC CTGCTGATGA ACCTGCAGAA AAGGCTGATG
841AACCAATGGA ACATTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
901tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)