Construct: ORF ccsbBroadEn_01593

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001467.1_s300c1, BRDN0000397474
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRP14 (6727)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6727 SRP14 signal recognition particle 14 NM_003134.6 100% 100%
2 human 6727 SRP14 signal recognition particle 14 NM_001309434.1 50% 44.9% 0_1ins184;26_65del
3 mouse 20813 Srp14 signal recognition particle 14 NM_009273.4 70.5% 72% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
474
ORF length:
408
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGT GTTGTTGGAG AGCGAGCAGT TCCTGACGGA GCTGACCAGA CTTTTCCAGA
121AGTGCCGGAC GTCGGGCAGC GTCTATATCA CCTTGAAGAA GTATGACGGT CGAACCAAAC
181CCATTCCAAA GAAGGGTACT GTGGAGGGCT TTGAGCCCGC AGACAACAAG TGTCTGTTAA
241GAGCTACCGA TGGGAAGAAG AAGATCAGCA CTGTGGTGAG CTCCAAGGAA GTGAATAAGT
301TTCAGATGGC TTATTCAAAC CTCCTTAGAG CTAACATGGA TGGGCTGAAG AAGAGAGACA
361AAAAGAACAA AACTAAGAAG ACCAAAGCAG CAGCAGCAGC AGCAGCAGCA GCACCTGCCG
421CAGCAGCAAC AGCACCAACA ACAGCAGCAA CAACAGCAGC AACAGCAGCA CAGTACCCAA
481CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)