Construct: ORF ccsbBroadEn_01939

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006979.1_s300c1, BRDN0000397430
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RGS5 (8490)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8490 RGS5 regulator of G protein sign... NM_003617.4 100% 100%
2 human 8490 RGS5 regulator of G protein sign... NM_001254749.2 97.8% 97.8% 384_395del
3 human 8490 RGS5 regulator of G protein sign... NM_001195303.3 40.3% 40.3% 0_1ins324
4 human 8490 RGS5 regulator of G protein sign... NM_001254748.1 40.3% 40.3% 0_1ins324
5 mouse 19737 Rgs5 regulator of G-protein sign... NM_009063.4 89.8% 90% (many diffs)
6 mouse 19737 Rgs5 regulator of G-protein sign... NM_001313705.1 35.7% 34.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
609
ORF length:
543
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTG CAAAGGACTT GCAGCTTTGC CCCACTCATG CCTGGAAAGG GCCAAGGAGA
121TTAAGATCAA GTTGGGAATT CTCCTCCAGA AGCCAGACTC AGTTGGTGAC CTTGTCATTC
181CGTACAATGA GAAGCCAGAG AAACCAGCCA AGACCCAGAA AACCTCGCTG GACGAGGCCC
241TGCAGTGGCG TGATTCCCTG GACAAACTCC TGCAGAACAA CTATGGACTT GCCAGTTTCA
301AAAGTTTCCT GAAGTCTGAA TTCAGTGAGG AAAACCTTGA GTTCTGGATT GCCTGTGAGG
361ATTACAAGAA GATCAAGTCC CCTGCCAAGA TGGCTGAGAA GGCAAAGCAA ATTTATGAAG
421AATTCATTCA AACGGAGGCT CCTAAAGAGG TGAATATTGA CCACTTCACT AAGGACATCA
481CAATGAAGAA CCTGGTGGAA CCTTCCCTGA GCAGCTTTGA CATGGCCCAG AAAAGAATCC
541ATGCCCTGAT GGAAAAGGAT TCTCTGCCTC GCTTTGTGCG CTCTGAGTTT TATCAGGAGT
601TAATCAAGTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa
661tttgttgcaa cgaac

Download FASTA (ORF) (Full)