Construct: ORF ccsbBroadEn_02162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011082.1_s300c1, BRDN0000387223
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCR1 (9437)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9437 | NCR1 | natural cytotoxicity trigge... | NM_001145457.3 | 99.7% | 99.6% | 244C>A;553A>C |
| 2 | human | 9437 | NCR1 | natural cytotoxicity trigge... | NM_004829.7 | 99.4% | 99.3% | 244C>A;553A>C;682_684delGCA |
| 3 | human | 9437 | NCR1 | natural cytotoxicity trigge... | NM_001145458.3 | 94.4% | 94.3% | 244C>A;553A>C;681_682ins48 |
| 4 | human | 9437 | NCR1 | natural cytotoxicity trigge... | XM_011527528.3 | 80% | 71.2% | (many diffs) |
| 5 | human | 9437 | NCR1 | natural cytotoxicity trigge... | XM_011527529.3 | 77% | 75.2% | (many diffs) |
| 6 | human | 9437 | NCR1 | natural cytotoxicity trigge... | XM_011527530.3 | 75.2% | 74.9% | (many diffs) |
| 7 | human | 9437 | NCR1 | natural cytotoxicity trigge... | NM_001242356.3 | 68.3% | 68.4% | 70_71ins285;268A>C;397_399delGCA |
| 8 | human | 9437 | NCR1 | natural cytotoxicity trigge... | XR_001753801.2 | 64% | (many diffs) | |
| 9 | human | 9437 | NCR1 | natural cytotoxicity trigge... | NM_001242357.3 | 63.2% | 63.3% | 70_71ins285;268A>C;396_397ins48 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC TTCCACACTC CCTGCCCTGC TCTGCGTCGG GCTGTGTCTG AGTCAGAGGA 121 TCAGCGCCCA GCAGCAGACT CTCCCAAAAC CGTTCATCTG GGCCGAGCCC CATTTCATGG 181 TTCCAAAGGA AAAGCAAGTG ACCATCTGTT GCCAGGGAAA TTATGGGGCT GTTGAATACC 241 AGCTGCACTT TGAAGGAAGC CTTTTTGCCG TGGACAGACC AAAACCCCCT GAGCGGATTA 301 ACAAAGTCAA ATTCTACATC CCGGACATGA ACTCCCGCAT GGCAGGGCAA TACAGCTGCA 361 TCTATCGGGT TGGGGAGCTC TGGTCAGAGC CCAGCAACTT GCTGGATCTG GTGGTAACAG 421 AAATGTATGA CACACCCACC CTCTCGGTTC ATCCTGGACC CGAAGTGATC TCGGGAGAGA 481 AGGTGACCTT CTACTGCCGT CTAGACACTG CAACAAGCAT GTTCTTACTG CTCAAGGAGG 541 GAAGATCCAG CCACGTACAG CGCGGATACG GGAAGGTCCA GGCGGAGTTC CCCCTGGGCC 601 CTGTGACCAC AGCCCACCGA GGGACATACC GATGTTTTGG CTCCTATAAC AACCATGCCT 661 GGTCTTTCCC CAGTGAGCCA GTGAAGCTCC TGGTCACAGG CGACATTGAG AACACCAGCC 721 TTGCACCTGA AGACCCCACC TTTCCTGACA CTTGGGGCAC CTACCTTTTA ACCACAGAGA 781 CGGGACTCCA GAAAGACCAT GCCCTCTGGG ATCACACTGC CCAGAATCTC CTTCGGATGG 841 GCCTGGCCTT TCTAGTCCTG GTGGCTCTAG TGTGGTTCCT GGTTGAAGAC TGGCTCAGCA 901 GGAAGAGGAC TAGAGAGCGA GCCAGCAGAG CTTCCACTTG GGAAGGCAGG AGAAGGCTGA 961 ACACACAGAC TCTTTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 1021 tatcaatttg ttgcaacgaa c