Construct: ORF ccsbBroadEn_02376
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005375.1_s300c1, BRDN0000388784
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAMP1 (10267)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10267 | RAMP1 | receptor activity modifying... | NM_005855.4 | 100% | 100% | |
| 2 | human | 10267 | RAMP1 | receptor activity modifying... | NM_001308353.2 | 85.1% | 85.1% | 0_1ins66 |
| 3 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003152.2 | 85.1% | 85.1% | 0_1ins66 |
| 4 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003153.2 | 85.1% | 85.1% | 0_1ins66 |
| 5 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003154.1 | 85.1% | 85.1% | 0_1ins66 |
| 6 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003156.2 | 85.1% | 85.1% | 0_1ins66 |
| 7 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003155.1 | 59.7% | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CCGGGCCCTG TGCCGCCTCC CGCGGCGCGG CCTCTGGCTG CTCCTGGCCC 121 ATCACCTCTT CATGACCACT GCCTGCCAGG AGGCTAACTA CGGTGCCCTC CTCCGGGAGC 181 TCTGCCTCAC CCAGTTCCAG GTAGACATGG AGGCCGTCGG GGAGACGCTG TGGTGTGACT 241 GGGGCAGGAC CATCAGGAGC TACAGGGAGC TGGCCGACTG CACCTGGCAC ATGGCGGAGA 301 AGCTGGGCTG CTTCTGGCCC AATGCAGAGG TGGACAGGTT CTTCCTGGCA GTGCATGGCC 361 GCTACTTCAG GAGCTGCCCC ATCTCAGGCA GGGCCGTGCG GGACCCGCCC GGCAGCATCC 421 TCTACCCCTT CATCGTGGTC CCCATCACGG TGACCCTGCT GGTGACGGCA CTGGTGGTCT 481 GGCAGAGCAA GCGCACTGAG GGCATTGTGT ACCCAACTTT CTTGTACAAA GTtggcatta 541 taagaaagca ttgcttatca atttgttgca acgaac