Construct: ORF ccsbBroadEn_03053

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000574.1_s300c1, BRDN0000389407
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM14A (28978)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 28978 TMEM14A transmembrane protein 14A NM_014051.4 100% 100%
2 human 28978 TMEM14A transmembrane protein 14A XM_024446409.1 100% 100%
3 mouse 75712 Tmem14a transmembrane protein 14A NM_029398.3 89.5% 92.9% (many diffs)
4 mouse 75712 Tmem14a transmembrane protein 14A XM_006495581.2 83.6% 86.7% (many diffs)
5 mouse 75712 Tmem14a transmembrane protein 14A NM_001290679.1 58.9% 52.9% (many diffs)
6 mouse 75712 Tmem14a transmembrane protein 14A XM_006495579.2 56.2% 50.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
363
ORF length:
297
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CCTGATCGGT TTTGGTTATG CAGCCCTCGT GACATTTGGA AGCATTTTTG
121GATATAAGCG GAGAGGTGGT GTTCCGTCTT TGATTGCTGG TCTTTTTGTT GGATGTTTGG
181CCGGCTATGG AGCTTACCGT GTCTCCAATG ACAAACGAGA TGTAAAAGTG TCACTGTTTA
241CAGCTTTCTT CCTGGCTACC ATAATGGGTG TGAGATTTAA GAGGTCCAAG AAAATAATGC
301CTGCTGGTTT GGTTGCAGGT TTAAGCCTCA TGATGATCCT GAGACTTGTC TTGTTGCTGC
361TCTGCCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
421gcaacgaac

Download FASTA (ORF) (Full)