Construct: ORF ccsbBroadEn_03129

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000045.1_s300c1, BRDN0000385361
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ERO1A (30001)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 30001 ERO1A endoplasmic reticulum oxido... NM_014584.3 100% 100%
2 human 30001 ERO1A endoplasmic reticulum oxido... XM_011536684.2 99.1% 99.1% 673_674insAAGGGACAAGTG
3 human 30001 ERO1A endoplasmic reticulum oxido... XM_024449554.1 94.1% 94.1% 1210_1296del
4 mouse 50527 Ero1l ERO1-like (S. cerevisiae) NM_015774.3 86.6% 91.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1470
ORF length:
1404
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG CCGCGGCTGG GGATTCTTGT TTGGCCTCCT GGGCGCCGTG TGGCTGCTCA
121GCTCGGGCCA CGGAGAGGAG CAGCCCCCGG AGACAGCGGC ACAGAGGTGC TTCTGCCAGG
181TTAGTGGTTA CTTGGATGAT TGTACCTGTG ATGTTGAAAC CATTGATAGA TTTAATAACT
241ACAGGCTTTT CCCAAGACTA CAAAAACTTC TTGAAAGTGA CTACTTTAGG TATTACAAGG
301TAAACCTGAA GAGGCCGTGT CCTTTCTGGA ATGACATCAG CCAGTGTGGA AGAAGGGACT
361GTGCTGTCAA ACCATGTCAA TCTGATGAAG TTCCTGATGG AATTAAATCT GCGAGCTACA
421AGTATTCTGA AGAAGCCAAT AATCTCATTG AAGAATGTGA ACAAGCTGAA CGACTTGGAG
481CAGTGGATGA ATCTCTGAGT GAGGAAACAC AGAAGGCTGT TCTTCAGTGG ACCAAGCATG
541ATGATTCTTC AGATAACTTC TGTGAAGCTG ATGACATTCA GTCCCCTGAA GCTGAATATG
601TAGATTTGCT TCTTAATCCT GAGCGCTACA CTGGTTACAA GGGACCAGAT GCTTGGAAAA
661TATGGAATGT CATCTACGAA GAAAACTGTT TTAAGCCACA GACAATTAAA AGACCTTTAA
721ATCCTTTGGC TTCTGGTCAA GGGACAAGTG AAGAGAACAC TTTTTACAGT TGGCTAGAAG
781GTCTCTGTGT AGAAAAAAGA GCATTCTACA GACTTATATC TGGCCTACAT GCAAGCATTA
841ATGTGCATTT GAGTGCAAGA TATCTTTTAC AAGAGACCTG GTTAGAAAAG AAATGGGGAC
901ACAACATTAC AGAATTTCAA CAGCGATTTG ATGGAATTTT GACTGAAGGA GAAGGTCCAA
961GAAGGCTTAA GAACTTGTAT TTTCTCTACT TAATAGAACT AAGGGCTTTA TCCAAAGTGT
1021TACCATTCTT CGAGCGCCCA GATTTTCAAC TCTTTACTGG AAATAAAATT CAGGATGAGG
1081AAAACAAAAT GTTACTTCTG GAAATACTTC ATGAAATCAA GTCATTTCCT TTGCATTTTG
1141ATGAGAATTC ATTTTTTGCT GGGGATAAAA AAGAAGCACA CAAACTAAAG GAGGACTTTC
1201GACTGCATTT TAGAAATATT TCAAGAATTA TGGATTGTGT TGGTTGTTTT AAATGTCGTC
1261TGTGGGGAAA GCTTCAGACT CAGGGTTTGG GCACTGCTCT GAAGATCTTA TTTTCTGAGA
1321AATTGATAGC AAATATGCCA GAAAGTGGAC CTAGTTATGA ATTCCATCTA ACCAGACAAG
1381AAATAGTATC ATTATTCAAC GCATTTGGAA GAATTTCTAC AAGTGTGAAA GAATTAGAAA
1441ACTTCAGGAA CTTGTTACAG AATATTCATT ACCCAACTTT CTTGTACAAA GTtggcatta
1501taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)