Construct: ORF ccsbBroadEn_03479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016672.1_s300c1, BRDN0000456670
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YTHDF1 (54915)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54915 | YTHDF1 | YTH N6-methyladenosine RNA ... | NM_017798.4 | 100% | 100% | |
| 2 | human | 54915 | YTHDF1 | YTH N6-methyladenosine RNA ... | XM_024451914.1 | 91% | 91% | 0_1ins150 |
| 3 | mouse | 228994 | Ythdf1 | YTH domain family 1 | NM_173761.3 | 88% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1743
- ORF length:
- 1677
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC GGCCACCAGC GTGGACACCC AGAGAACAAA AGGACAAGAT AATAAAGTAC 121 AAAATGGTTC GTTACATCAG AAGGATACAG TTCATGACAA TGACTTTGAG CCCTACCTTA 181 CTGGACAGTC AAATCAGAGT AACAGTTACC CCTCAATGAG CGACCCCTAC CTGTCCAGCT 241 ATTACCCGCC GTCCATTGGA TTTCCTTACT CCCTCAATGA GGCTCCGTGG TCTACTGCAG 301 GGGACCCTCC GATTCCATAC CTCACCACCT ACGGACAGCT CAGTAACGGA GACCATCATT 361 TTATGCACGA TGCTGTTTTT GGGCAGCCTG GGGGCCTGGG GAACAACATC TATCAGCACA 421 GGTTCAATTT TTTCCCTGAA AACCCTGCGT TCTCAGCATG GGGGACAAGT GGGTCTCAAG 481 GTCAGCAGAC CCAGAGCTCC GCGTATGGGA GCAGCTACAC CTACCCCCCG AGCTCCCTGG 541 GTGGCACGGT GGTTGATGGG CAGCCAGGCT TTCACAGCGA CACCCTCAGC AAGGCCCCCG 601 GGATGAACAG CCTGGAGCAG GGCATGGTTG GCCTGAAGAT TGGGGACGTC AGCTCCTCCG 661 CCGTCAAGAC GGTGGGCTCT GTCGTCAGCA GCGTGGCACT GACTGGTGTC CTTTCTGGCA 721 ACGGTGGGAC AAATGTGAAC ATGCCAGTTT CAAAGCCGAC CTCGTGGGCT GCCATTGCCA 781 GCAAGCCTGC AAAACCACAG CCTAAAATGA AAACAAAGAG CGGGCCTGTC ATGGGGGGTG 841 GGCTGCCCCC TCCACCCATA AAGCATAACA TGGACATTGG CACCTGGGAT AACAAGGGGC 901 CTGTGCCGAA GGCCCCAGTC CCCCAGCAGG CACCCTCTCC ACAGGCTGCC CCACAGCCCC 961 AGCAGGTGGC TCAGCCTCTC CCAGCACAGC CCCCAGCTTT GGCTCAACCG CAGTATCAGA 1021 GCCCTCAGCA GCCACCCCAG ACCCGCTGGG TTGCCCCACG CAACAGAAAC GCGGCGTTTG 1081 GGCAGAGCGG AGGGGCTGGC AGCGATAGCA ACTCTCCTGG AAACGTCCAG CCTAATTCTG 1141 CCCCCAGCGT CGAATCCCAC CCCGTCCTTG AAAAACTGAA GGCTGCTCAC AGCTACAACC 1201 CGAAAGAGTT TGAGTGGAAT CTGAAAAGCG GGCGTGTGTT CATCATCAAG AGCTACTCTG 1261 AGGACGACAT CCACCGCTCC ATTAAGTACT CCATCTGGTG TAGCACAGAG CACGGCAACA 1321 AGCGCCTGGA CAGCGCCTTC CGCTGCATGA GCAGCAAGGG GCCCGTCTAC CTGCTCTTCA 1381 GCGTCAATGG GAGTGGGCAT TTTTGTGGGG TGGCCGAGAT GAAGTCCCCC GTGGACTACG 1441 GCACCAGTGC CGGGGTCTGG TCTCAGGACA AGTGGAAGGG GAAGTTTGAT GTCCAGTGGA 1501 TTTTTGTTAA GGATGTACCC AATAACCAGC TCCGGCACAT CAGGCTGGAG AATAACGACA 1561 ACAAACCGGT CACAAACTCC CGGGACACCC AGGAGGTGCC CTTAGAAAAA GCCAAGCAAG 1621 TGCTGAAAAT TATCAGTTCC TACAAGCACA CAACCTCCAT CTTCGACGAC TTTGCTCACT 1681 ACGAGAAGCG CCAGGAGGAG GAGGAGGTGG TGCGCAAGGA ACGGCAGAGT CGAAACAAAC 1741 AATGCCCAAC TTTCTTGTAC AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt 1801 gcaacgaac