Construct: ORF ccsbBroadEn_03627

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010381.1_s300c1, BRDN0000393786
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RUFY2 (55680)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55680 RUFY2 RUN and FYVE domain contain... NM_001042417.1 100% 100%
2 human 55680 RUFY2 RUN and FYVE domain contain... NM_001278225.1 78.7% 78.4% 0_1ins174;2_3delTGinsAA;123_224del
3 human 55680 RUFY2 RUN and FYVE domain contain... XM_005269957.5 68.3% 65.5% (many diffs)
4 human 55680 RUFY2 RUN and FYVE domain contain... NM_001330103.2 64.5% 61.7% (many diffs)
5 human 55680 RUFY2 RUN and FYVE domain contain... XM_011539942.2 64.5% 61.7% (many diffs)
6 human 55680 RUFY2 RUN and FYVE domain contain... XM_005269953.4 64.3% 61.6% (many diffs)
7 human 55680 RUFY2 RUN and FYVE domain contain... XM_005269955.4 61.8% 59% (many diffs)
8 human 55680 RUFY2 RUN and FYVE domain contain... NM_017987.4 60.9% 58.1% (many diffs)
9 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747132.2 56.8% (many diffs)
10 human 55680 RUFY2 RUN and FYVE domain contain... NR_103476.2 54.5% (many diffs)
11 human 55680 RUFY2 RUN and FYVE domain contain... XM_005269956.4 52.2% 49.4% (many diffs)
12 human 55680 RUFY2 RUN and FYVE domain contain... NR_103475.2 48.2% (many diffs)
13 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747130.2 27.7% (many diffs)
14 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747131.2 27.3% (many diffs)
15 human 55680 RUFY2 RUN and FYVE domain contain... XR_246097.2 25.3% (many diffs)
16 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747134.1 21.6% (many diffs)
17 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747129.1 20.9% (many diffs)
18 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747133.1 20.9% 1_113del;1323_5780del
19 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747127.2 20.6% 1_96del;393_494del;1408_5865del
20 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747125.1 20.1% 1_235delinsA;531_632del;1546_6003del
21 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747126.1 19.8% 1_433delinsA;1642_6099del
22 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747128.1 19.3% 1_489del;786_887del;1801_6258del
23 human 55680 RUFY2 RUN and FYVE domain contain... XR_001747135.2 17.7% (many diffs)
24 mouse 70432 Rufy2 RUN and FYVE domain-contain... XM_017314087.1 79.8% 88.5% (many diffs)
25 mouse 70432 Rufy2 RUN and FYVE domain-contain... XM_006514089.3 73.8% 81.9% (many diffs)
26 mouse 70432 Rufy2 RUN and FYVE domain-contain... XM_006514088.1 66% 69.6% (many diffs)
27 mouse 70432 Rufy2 RUN and FYVE domain-contain... NM_027425.3 56.7% 59.7% (many diffs)
28 mouse 70432 Rufy2 RUN and FYVE domain-contain... XM_006514087.1 56.5% 59.5% (many diffs)
29 mouse 70432 Rufy2 RUN and FYVE domain-contain... XM_011243562.2 54.3% 57% (many diffs)
30 mouse 70432 Rufy2 RUN and FYVE domain-contain... XR_001779596.1 40.4% (many diffs)
31 mouse 70432 Rufy2 RUN and FYVE domain-contain... XR_001779594.1 24.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1275
ORF length:
1209
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TACAAAAGAC CCCACAGCTG TAGAGAGAGC AAACTTGTTA AACATGGCTA
121AACTGAGTAT CAAAGGACTC ATTGAATCTG CTCTGAGCTT TGGCCGCACT TTGGATTCTG
181ACTATCCCCC CTTGCAGCAA TTCTTTGTTG TTATGGAACA TTGCCTGAAA CACGGTCTTA
241AAGTAAGAAA ATCATTTTTG AGTTACAACA AAACCATCTG GGGCCCTTTG GAACTGGTGG
301AGAAGCTGTA CCCCGAAGCA GAGGAAATAG GAGCTAGTGT CCGGGATCTA CCTGGTCTGA
361ATGAGTTTTA TGAGTATCAC GCACTAATGA TGGAAGAAGA AGGAGCAGTA ATTGTTGGGC
421TGCTGGTTGG CCTGAATGTG ATCGATGCTA ATCTGTGTGT GAAGGGAGAG GATTTAGACT
481CACAAGTTGG AGTGATTGAT TTTTCTATGT ATTTAAAGAA TGAAGAAGAT ATTGGAAATA
541AAGAAAGGAA TGTTCAAATT GCTGCCATAT TAGACCAAAA GAATTATGTT GAAGAATTAA
601ATAGACAACT GAACAGCACA GTCAGCAGCC TCCATTCAAG AGTTGATTCA TTAGAAAAGT
661CAAATACTAA GCTGATTGAA GAGTTAGCAA TAGCAAAGAA TAACATCATT AAACTCCAGG
721AAGAAAATCA TCAATTACGA AGTGAAAATA AATTGATTTT AATGAAAACA CAGCAGCACC
781TAGAGGTTAC CAAAGTAGAT GTGGAAACTG AGCTTCAAAC ATATAAGCAT TCTCGTCAGG
841GGCTAGATGA AATGTACAAT GAAGCCAGAA GGCAGCTTCG AGATGAATCT CAGTTACGAC
901AGGATGTAGA GAATGAGCTA GCAGTACAAG TTAGTATGAA GCATGAGATT GAACTTGCCA
961TGAAGTTGCT GGAGAAAGAT ATCCATGAGA AACAAGATAC TCTGATAGGC CTTCGACAAC
1021AACTAGAGGA AGTTAAAGCA ATTAACATAG AGATGTATCA AAAGTTGCAG GGTTCTGAAG
1081ATGGCTTGAA AGAAAAAAAT GAAATAATTG CCCGACTAGA AGAAAAAACC AATAAAATTA
1141CTGCAGCCAT GAGGCAGCTG GAACAAAGTG ACAACGATTT GTTAACTCAA ACTAGGACAA
1201TTGCAATGTC ATTGGTGAAA TGTGCCAGCA GTGACACGCA GGACCAGTAC AAGCTGGTTA
1261AAGATATATC TTTCTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
1321tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)

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