Construct: ORF ccsbBroadEn_03692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012463.1_s300c1, BRDN0000397586
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFC1 (55997)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55997 | CFC1 | cripto, FRL-1, cryptic fami... | NM_032545.3 | 100% | 100% | |
| 2 | human | 653275 | CFC1B | cripto, FRL-1, cryptic fami... | NM_001079530.2 | 99.7% | 99.5% | 225A>C;232T>C |
| 3 | human | 55997 | CFC1 | cripto, FRL-1, cryptic fami... | NM_001270420.1 | 80.5% | 35% | 246_247ins115;555_573del |
| 4 | human | 653275 | CFC1B | cripto, FRL-1, cryptic fami... | XM_005263737.4 | 80.2% | 34.7% | (many diffs) |
| 5 | human | 55997 | CFC1 | cripto, FRL-1, cryptic fami... | NM_001270421.1 | 66.3% | 66.3% | 246_247ins225 |
| 6 | human | 55997 | CFC1 | cripto, FRL-1, cryptic fami... | XM_011511486.3 | 47.2% | 34.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAC CTGGAGGCAC CATGTCAGGC TTCTGTTTAC GGTCAGTTTG GCATTACAGA 121 TCATCAATTT GGGAAACAGC TATCAAAGAG AGAAACATAA CGGCGGTAGA GAGGAAGTCA 181 CCAAGGTTGC CACTCAGAAG CACCGACAGT CACCGCTCAA CTGGACCTCC AGTCATTTCG 241 GAGAGGTGAC TGGGAGCGCC GAGGGCTGGG GGCCGGAGGA GCCGCTCCCC TACTCCCGGG 301 CTTTCGGAGA GGGTGCGTCC GCGCGGCCGC GCTGCTGCAG GAACGGCGGT ACCTGCGTGC 361 TGGGCAGCTT CTGCGTGTGC CCGGCCCACT TCACCGGCCG CTACTGCGAG CATGACCAGA 421 GGCGCAGTGA ATGCGGCGCC CTGGAGCACG GAGCCTGGAC CCTCCGCGCC TGCCACCTCT 481 GCAGGTGCAT CTTCGGGGCC CTGCACTGCC TCCCCCTCCA GACGCCTGAC CGCTGTGACC 541 CGAAAGACTT CCTGGCCTCC CACGCTCACG GGCCGAGCGC CGGGGGCGCG CCCAGCCTGC 601 TACTCTTGCT GCCCTGCGCA CTCCTGCACC GCCTCCTGCG CCCGGATGCG CCCGCGCACC 661 CTCGGTCCCT GGTCCCTTCC GTCCTCCAGC GGGAGCGGCG CCCCTGCGGA AGGCCGGGAC 721 TTGGGCATCG CCTTTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 781 tatcaatttg ttgcaacgaa c