Construct: ORF ccsbBroadEn_03816

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011311.1_s300c1, BRDN0000390114
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPC25 (57405)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57405 SPC25 SPC25 component of NDC80 ki... NM_020675.4 100% 100%
2 human 57405 SPC25 SPC25 component of NDC80 ki... XM_011511516.2 82.4% 82.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
738
ORF length:
672
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGT AGAGGACGAA CTGGCACTTT TCGATAAAAG CATAAATGAA TTTTGGAATA
121AATTCAAAAG TACGGACACC TCCTGTCAGA TGGCGGGACT AAGAGATACC TACAAGGATT
181CCATCAAAGC ATTTGCAGAA AAGCTGTCTG TGAAATTAAA GGAAGAAGAA CGAATGGTTG
241AGATGTTTCT GGAATATCAA AATCAGATCA GCAGGCAAAA TAAGCTCATT CAAGAAAAAA
301AGGATAACTT GTTAAAATTG ATTGCTGAAG TAAAAGGCAA AAAGCAGGAA TTGGAAGTAC
361TGACTGCAAA TATCCAGGAT CTTAAGGAAG AATATTCTAG GAAGAAGGAA ACTATTTCTA
421CTGCTAATAA AGCGAATGCA GAGAGGTTGA AAAGGCTGCA GAAATCTGCA GACTTGTATA
481AAGATCGACT TGGACTAGAA ATTCGAAAAA TTTATGGTGA GAAATTGCAG TTTATTTTCA
541CTAATATTGA CCCTAAGAAT CCTGAGAGCC CATTTATGTT TTCCTTACAT CTCAATGAAG
601CAAGGGACTA TGAAGTGTCA GATAGTGCCC CTCATCTTGA GGGCCTAGCA GAATTTCAAG
661AGAATGTAAG GAAGACCAAC AATTTTTCAG CTTTTCTTGC CAATGTTCGG AAAGCTTTTA
721CTGCCACGGT TTATAATTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
781gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)