Construct: ORF ccsbBroadEn_03896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009146.1_s300c1, BRDN0000455527
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG101 (60673)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60673 | ATG101 | autophagy related 101 | NM_001098673.2 | 100% | 100% | |
| 2 | human | 60673 | ATG101 | autophagy related 101 | NM_021934.4 | 100% | 100% | |
| 3 | human | 60673 | ATG101 | autophagy related 101 | XM_024449120.1 | 100% | 100% | |
| 4 | mouse | 68118 | Atg101 | autophagy related 101 | NM_026566.2 | 91.1% | 98.6% | (many diffs) |
| 5 | mouse | 68118 | Atg101 | autophagy related 101 | XM_006521310.3 | 91.1% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA CTGTCGCTCG GAGGTGCTGG AGGTGTCGGT GGAGGGGCGG CAGGTGGAGG 121 AGGCCATGCT GGCTGTGCTG CACACGGTGC TTCTGCACCG CAGCACAGGC AAGTTCCACT 181 ACAAGAAGGA GGGCACCTAC TCCATTGGCA CCGTGGGCAC CCAGGATGTT GACTGTGACT 241 TCATCGACTT CACTTATGTG CGTGTCTCTT CTGAGGAACT GGATCGTGCC CTGCGCAAGG 301 TTGTTGGGGA GTTCAAGGAT GCACTGCGCA ACTCTGGTGG CGATGGGCTG GGGCAGATGT 361 CCTTGGAGTT CTACCAGAAG AAGAAGTCTC GCTGGCCATT CTCAGACGAG TGCATCCCAT 421 GGGAAGTGTG GACGGTCAAG GTGCATGTGG TAGCCCTGGC CACGGAGCAG GAGCGGCAGA 481 TCTGCCGGGA GAAGGTGGGT GAGAAACTCT GCGAGAAGAT CATCAACATC GTGGAGGTGA 541 TGAATCGGCA TGAGTACTTG CCCAAGATGC CCACACAGTC GGAGGTGGAT AACGTGTTTG 601 ACACAGGCTT GCGGGACGTG CAGCCCTACC TGTACAAGAT CTCCTTCCAG ATCACTGATG 661 CCCTGGGCAC CTCAGTCACC ACCACCATGC GCAGGCTCAT CAAAGACACC CTTGCCCTCT 721 GCCCAACTTT CTTGTACAAA GTTGGcatta taagaaagca ttgcttatca atttgttgca 781 acgaac