Construct: ORF ccsbBroadEn_03991
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008433.1_s300c1, BRDN0000456963
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS9 (64965)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64965 | MRPS9 | mitochondrial ribosomal pro... | NM_182640.3 | 100% | 100% | |
| 2 | human | 64965 | MRPS9 | mitochondrial ribosomal pro... | XM_011511644.2 | 68.6% | 68.6% | 0_1ins372 |
| 3 | human | 64965 | MRPS9 | mitochondrial ribosomal pro... | XR_001738903.2 | 47% | 1_624del;1197_1705del;2322_2523del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1257
- ORF length:
- 1188
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCGGCGCCC TGTGTGTCCT ACGGCGGAGC AGTTTCGTAC CGGCTTCTTC 121 TCTGGGGTAG GGGTAGCCTC GCCCGGAAGC AAGGCCTCTG GAAAACCGCG GCCCCTGAGT 181 TGCAAACAAA TGTCAGATCC CAGATATTAA GGCTAAGACA TACTGCATTT GTAATACCAA 241 AGAAAAACGT TCCTACCTCA AAACGTGAAA CTTACACAGA GGATTTTATT AAAAAGCAGA 301 TTGAAGAGTT CAACATAGGA AAGAGACATT TAGCCAACAT GATGGGAGAA GATCCAGAAA 361 CTTTCACTCA AGAAGATATT GACAGAGCTA TTGCTTACCT TTTCCCAAGT GGTTTGTTTG 421 AGAAACGAGC CAGGCCAGTA ATGAAGCATC CTGAACAGAT TTTTCCAAGA CAAAGAGCAA 481 TCCAGTGGGG AGAAGATGGC CGTCCATTTC ACTATCTCTT CTATACTGGC AAACAGTCAT 541 ACTATTCATT AATGCATGAT GTATATGGAA TGTTACTCAA TTTAGAAAAA CATCAAAGTC 601 ACTTGCAAGC CAAAAGTCTG CTCCCAGAAA AAACTGTAAC CAGAGACGTG ATTGGCAGCA 661 GATGGCTGAT TAAGGAGGAA CTAGAAGAAA TGTTAGTGGA AAAACTGTCA GATCTAGATT 721 ATATGCAGTT CATTCGGCTG CTAGAAAAGT TATTGACATC GCAGTGTGGT GCTGCTGAGG 781 AAGAATTTGT GCAGAGGTTT CGAAGAAGTG TAACTCTTGA ATCAAAAAAA CAGCTGATTG 841 AACCTGTACA GTATGATGAG CAAGGAATGG CCTTTAGCAA AAGTGAAGGT AAAAGAAAGA 901 CTGCAAAAGC AGAAGCAATT GTTTATAAAC ATGGAAGTGG AAGAATAAAA GTAAATGGAA 961 TTGATTACCA GCTTTACTTC CCGATCACAC AGGACAGAGA ACAGCTGATG TTCCCTTTCC 1021 ACTTTGTTGA CCGGCTGGGA AAGCACGACG TGACCTGCAC AGTCTCAGGG GGCGGGAGGT 1081 CAGCGCAGGC TGGAGCAATA CGACTGGCAA TGGCAAAAGC CTTGTGCAGC TTTGTCACCG 1141 AGGACGAGGT CGAGTGGATG AGACAAGCTG GACTACTTAC TACTGATCCA CGTGTGAGGG 1201 AACGGAAGAA GCCAGGCCAA GAGGGAGCCC GCAGAAAGTT TACGTGGAAG AAACGCTTGC 1261 CAACTTTCTT GTACAAAGTT GGcattataa gaaagcattg cttatcaatt tgttgcaacg 1321 aac