Construct: ORF ccsbBroadEn_04202

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002198.1_s300c1, BRDN0000395548
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MED28 (80306)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80306 MED28 mediator complex subunit 28 NM_025205.5 100% 100%
2 mouse 66999 Med28 mediator complex subunit 28 NM_025895.4 88% 94.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
600
ORF length:
534
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCTCCACTA GGGGGTATGT TTTCTGGGCA GCCACCCGGT CCCCCTCAGG
121CCCCGCCGGG CCTTCCGGGC CAAGCTTCGC TTCTTCAGGC AGCTCCAGGC GCTCCTAGAC
181CTTCCAGCAG TACTTTGGTG GACGAGTTGG AGTCATCTTT CGAGGCTTGC TTTGCATCTC
241TGGTGAGTCA GGACTATGTC AATGGCACCG ATCAGGAAGA AATTCGAACC GGTGTTGATC
301AGTGTATCCA GAAGTTTCTG GATATTGCAA GACAGACAGA ATGTTTTTTC TTACAAAAAA
361GATTGCAGTT ATCTGTCCAG AAACCAGAGC AAGTTATCAA AGAGGATGTG TCAGAACTAA
421GGAATGAATT ACAGCGGAAA GATGCACTAG TCCAGAAGCA CTTGACAAAG CTGAGGCATT
481GGCAGCAGGT GCTGGAGGAC ATCAACGTGC AGCACAAAAA GCCCGCCGAC ATCCCTCAGG
541GCTCCTTGGC CTACCTGGAG CAGGCATCTG CCAACATCCC TGCACCTCTG AAGCCAACGT
601GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
661acgaac

Download FASTA (ORF) (Full)