Construct: ORF ccsbBroadEn_04273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002563.1_s300c1, BRDN0000391110
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOHH (83475)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83475 | DOHH | deoxyhypusine hydroxylase | NM_001145165.2 | 100% | 100% | |
| 2 | human | 83475 | DOHH | deoxyhypusine hydroxylase | NM_031304.5 | 100% | 100% | |
| 3 | human | 83475 | DOHH | deoxyhypusine hydroxylase | XM_011528341.2 | 47% | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 973
- ORF length:
- 906
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG GTACAAAAAA 61 GTTTGCATGG TGACGGAGCA GGAGGTGGAT GCCATCGGGC AGACGCTGGT GGACCCCAAG 121 CAGCCCCTGC AGGCCCGCTT CCGGGCGCTG TTCACGCTGC GTGGGCTCGG CGGCCCAGGC 181 GCCATTGCAT GGATCAGCCA GGCCTTCGAT GACGATTCCG CCCTGCTCAA GCACGAGCTG 241 GCCTACTGCC TGGGCCAGAT GCAGGATGCC CGCGCCATCC CCATGCTGGT GGACGTGCTG 301 CAAGACACCC GTCAGGAGCC CATGGTGCGC CATGAGGCAG GGGAGGCCCT GGGGGCCATC 361 GGGGACCCGG AAGTTCTGGA GATCCTGAAG CAGTATTCCT CGGACCCCGT CATCGAGGTG 421 GCCGAGACCT GCCAGCTGGC CGTGCGCAGG CTGGAGTGGC TGCAGCAGCA CGGCGGGGAG 481 CCGGCGGCGG GACCCTACCT CTCCGTGGAC CCTGCCCCGC CGGCTGAGGA GCGTGACGTG 541 GGGCGCCTGC GGGAGGCGCT GCTGGATGAG TCCCGGCCGC TCTTCGAGCG ATACCGCGCC 601 ATGTTCGCCC TGCGCAACGC GGGAGGCGAG GAGGCCGCCC TGGCGCTGGC CGAGGGTCTG 661 CACTGTGGGA GCGCCCTCTT CCGCCACGAG GTCGGCTACG TCCTGGGACA GCTGCAGCAC 721 GAGGCGGCGG TGCCCCAGCT GGCGGCCGCC CTGGCCCGAT GCACCGAGAA CCCCATGGTG 781 CGGCACGAGT GCGCGGAGGC CCTGGGCGCC ATTGCCCGGC CCGCCTGCCT GGCCGCGCTG 841 CAGGCTCACG CGGACGACCC AGAGCGCGTG GTGCGTGAGA GCTGCGAGGT GGCTCTGGAC 901 ATGTATGAGC ACGAGACCGG GCGGGCCTTC CAGTACGCGG ACGGCCTGGA GCAGCTGCGC 961 GGGGCCCCCT CCTACCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta 1021 tcaatttgtt gcaacgaac