Construct: ORF ccsbBroadEn_04434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017949.1_s300c1, BRDN0000396712
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL22 (84861)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84861 | KLHL22 | kelch like family member 22 | NM_032775.4 | 100% | 100% | |
| 2 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029021.2 | 100% | 100% | |
| 3 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029020.2 | 95.1% | 95.1% | 1_96del |
| 4 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029019.2 | 92.9% | 92.9% | 1_144del |
| 5 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029024.1 | 89.1% | 78.4% | 1_46del;267_268ins166 |
| 6 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029023.1 | 88.3% | 81.2% | 1_64del;285_286ins166 |
| 7 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029025.1 | 87.8% | 87.8% | 0_1ins231 |
| 8 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029022.1 | 86.1% | 80.9% | 1_112del;333_334ins166 |
| 9 | human | 84861 | KLHL22 | kelch like family member 22 | XM_017029018.2 | 76.4% | 66.7% | 1_370del;591_592ins166 |
| 10 | human | 84861 | KLHL22 | kelch like family member 22 | XR_001755341.2 | 72.7% | (many diffs) | |
| 11 | human | 84861 | KLHL22 | kelch like family member 22 | NR_033825.2 | 68.6% | 1_69del;290_291ins166;1806_2362del | |
| 12 | mouse | 224023 | Klhl22 | kelch-like 22 | NM_001359068.1 | 87.2% | 94.6% | (many diffs) |
| 13 | mouse | 224023 | Klhl22 | kelch-like 22 | NM_145479.4 | 87.2% | 94.6% | (many diffs) |
| 14 | mouse | 224023 | Klhl22 | kelch-like 22 | XM_006522041.4 | 87.2% | 94.6% | (many diffs) |
| 15 | mouse | 224023 | Klhl22 | kelch-like 22 | XM_006522039.3 | 84.7% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1968
- ORF length:
- 1902
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG GACAAAAAAG 61 TTGGCATGGC AGAGGAGCAG GAGTTCACCC AGCTCTGCAA GTTGCCTGCA CAGCCCTCAC 121 ACCCACACTG CGTGAACAAC ACCTACCGCA GCGCACAGCA CTCCCAGGCT CTGCTCCGAG 181 GGCTGCTGGC TCTCCGGGAC AGCGGAATCC TCTTCGATGT TGTGCTGGTG GTGGAGGGCA 241 GACACATCGA GGCCCATCGC ATCCTGCTGG CTGCGTCCTG CGATTACTTC AGAGGAATGT 301 TTGCTGGGGG ATTGAAGGAG ATGGAACAGG AAGAGGTCCT GATCCACGGT GTGTCCTACA 361 ATGCTATGTG CCAAATCCTA CATTTCATAT ACACCTCCGA GCTGGAGCTC AGCCTGAGCA 421 ATGTACAAGA GACACTGGTG GCTGCCTGCC AGCTTCAGAT CCCAGAAATT ATCCATTTCT 481 GCTGTGATTT CCTCATGTCC TGGGTGGACG AAGAGAACAT TCTCGATGTC TACCGGCTGG 541 CAGAGCTGTT TGACTTGAGC CGCCTGACTG AGCAACTGGA CACCTATATC CTCAAAAACT 601 TTGTGGCCTT CTCTCGGACT GACAAGTACC GCCAGCTTCC ATTGGAGAAG GTCTACTCCC 661 TCCTCAGCAG CAATCGCCTG GAGGTCTCCT GCGAGACCGA GGTATATGAG GGGGCCCTTC 721 TCTACCATTA TAGCCTGGAG CAGGTGCAGG CTGACCAGAT CTCGCTGCAC GAGCCCCCAA 781 AGCTCCTTGA GACAGTGCGG TTTCCGCTGA TGGAAGCTGA GGTCCTGCAG CGGCTGCATG 841 ACAAGCTGGA CCCCAGCCCT TTGAGGGACA CAGTGGCCAG CGCCCTCATG TACCACCGGA 901 ACGAGAGCCT ACAGCCCAGC CTGCAGAGCC CGCAAACGGA GCTGCGGTCG GACTTCCAGT 961 GCGTTGTGGG CTTCGGGGGC ATTCACTCCA CGCCGTCCAC TGTCCTCAGC GACCAGGCCA 1021 AGTATCTAAA CCCCTTACTG GGAGAGTGGA AGCACTTCAC TGCCTCCCTG GCCCCCCGCA 1081 TGTCCAACCA GGGCATCGCG GTGCTCAACA ACTTCGTATA CTTGATTGGA GGGGACAACA 1141 ATGTCCAAGG ATTTCGAGCA GAGTCCCGAT GCTGGAGGTA TGACCCACGG CACAACCGCT 1201 GGTTCCAGAT CCAGTCCCTG CAGCAGGAGC ACGCCGACCT GTCCGTGTGT GTTGTAGGCA 1261 GGTACATCTA CGCTGTGGCG GGCCGTGACT ACCACAATGA CCTGAATGCT GTGGAGCGCT 1321 ACGACCCTGC CACCAACTCC TGGGCATACG TGGCCCCACT CAAGAGGGAG GTGTATGCCC 1381 ACGCAGGCGC GACGCTGGAG GGGAAGATGT ATATCACCTG CGGCCGCAGA GGGGAGGATT 1441 ACCTGAAAGA GACACACTGC TACGATCCAG GCAGCAACAC TTGGCACACA CTGGCTGATG 1501 GGCCTGTGCG GCGCGCCTGG CACGGCATGG CAACCCTCCT CAACAAGCTG TATGTGATCG 1561 GGGGCAGCAA CAACGATGCC GGATACAGGA GGGACGTGCA CCAGGTGGCC TGCTACAGCT 1621 GCACGTCTGG ACAGTGGTCA TCTGTCTGCC CACTCCCTGC TGGGCACGGT GAGCCTGGCA 1681 TTGCTGTGCT GGACAACAGG ATCTATGTGT TAGGTGGCCG CTCACACAAC CGCGGCAGCC 1741 GCACAGGCTA CGTGCACATT TACGATGTGG AGAAGGACTG CTGGGAGGAA GGGCCCCAGC 1801 TGGACAACTC CATCTCAGGC CTGGCGGCCT GTGTGCTCAC CCTGCCCCGC TCCCTGCTCC 1861 TTGAGCCGCC CCGCGGGACC CCTGACCGCA GCCAGGCCGA CCCGGACTTT GCCTCTGAGG 1921 TGATGAGTGT GTCTGACTGG GAGGAGTTTG ACAACTCCAG TGAGGACTAC CCAACTTTCT 1981 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac