Construct: ORF ccsbBroadEn_04609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014765.1_s300c1, BRDN0000384983
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5IF1 (93974)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_016311.5 | 100% | 100% | |
| 2 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_178190.3 | 63.2% | 61.3% | (many diffs) |
| 3 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_178191.2 | 56.2% | 56.6% | 180_180delGins139 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 384
- ORF length:
- 318
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AGTGACGGCG TTGGCGGCGC GGACGTGGCT TGGCGTGTGG GGCGTGAGGA 121 CCATGCAAGC CCGAGGCTTC GGCTCGGATC AGTCCGAGAA TGTCGACCGG GGCGCGGGCT 181 CCATCCGGGA AGCCGGTGGG GCCTTCGGAA AGAGAGAGCA GGCTGAAGAG GAACGATATT 241 TCCGAGCACA GAGTAGAGAA CAACTGGCAG CTTTGAAAAA ACACCATGAA GAAGAAATCG 301 TTCATCATAA GAAGGAGATT GAGCGTCTGC AGAAAGAAAT TGAGCGCCAT AAGCAGAAGA 361 TCAAAATGCT AAAACATGAT GATTACCCAA CTTTCTTGTA CAAAGTtggc attataagaa 421 agcattgctt atcaatttgt tgcaacgaac