Construct: ORF ccsbBroadEn_05093

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003033.1_s300c1, BRDN0000457134
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RASEF (158158)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 158158 RASEF RAS and EF-hand domain cont... NM_152573.4 100% 100%
2 human 158158 RASEF RAS and EF-hand domain cont... XM_005251730.4 96.8% 96.8% 959_960ins69
3 human 158158 RASEF RAS and EF-hand domain cont... XM_005251731.4 82% 81.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2289
ORF length:
2220
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGAGGCGGAT GGGGACGGAG AGGAGCTGGC CCGGCTGCGC TCAGTCTTCG
121CCGCCTGCGA CGCGAACCGC TCGGGGCGCC TGGAGCGCGA GGAGTTCCGG GCACTGTGCA
181CGGAGCTGCG GGTGCGGCCG GCCGACGCCG AGGCAGTATT CCAGCGGCTG GACGCCGACC
241GTGACGGCGC CATCACCTTC CAGGAGTTCG CGCGTGGCTT CCTCGGGTCC CTCCGCGGGG
301GGCGGCGCCG GGACTGGGGT CCTCTGGATC CCGCGCCCGC CGTGTCTGAG GCGGGGCCGG
361AGACACACGA CAGCGAGGAG GACGAAGGCG ACGAGGACGC GGCGGCGGCG CTGGCCACCT
421CGTGCGGCCC GGCGAGTCCC GGCCGGGCTT GGCAGGATTT CCAGGCGCGA CTTGGGGACG
481AAGCCAAGTT CATTCCCAGA GAAGAGCAAG TTAGTACCTT GTACCAAAAC ATCAACCTTG
541TGGAGCCAAG ATTAATTCAG CCATATGAAC ATGTTATAAA GAACTTCATC CGTGAGATCA
601GACTTCAAAG CACAGAAATG GAAAATTTGG CCATTGCGGT GAAGAGAGCC CAGGACAAGG
661CAGCTATGCA GTTGAGTGAG TTGGAAGAGG AAATGGATCA GAGGATTCAG GCTGCAGAAC
721ATAAGACACG GAAAGACGAA AAACGCAAAG CTGAGGAAGC CCTCAGTGAC CTCAGACGTC
781AGTATGAAAC TGAAGTAGGA GATCTGCAGG TGACCATTAA AAAGCTAAGA AAGCTCGAAG
841AACAATCAAA ACGCGTAAGT CAAAAGGAAG ATGTGGCTGC ATTGAAAAAA CAAATTTATG
901ATTTATCAAT GGAAAACCAG AAAGTTAAGA AAGACCTTTT AGAAGCACAG ACAAACATAG
961CCTTTCTTCA GAGTGAGTTA GATGCTTTGA AAAGTGATTA TGCTGATCAG AGTCTGAATA
1021CTGAAAGGGA TCTGGAAATA ATCCGAGCAT ACACAGAAGA TCGAAATAGT CTTGAGAGGC
1081AAATTGAAAT ACTCCAAACA GCTAACCGGA AGCTACATGA CAGTAATGAT GGCCTTAGAA
1141GTGCCCTTGA AAACAGTTAT AGCAAGTTCA ACAGATCTTT GCATATAAAT AATATCTCAC
1201CAGGGAATAC AATTTCTAGA AGCAGTCCCA AATTCATTGG TCATTCCCCT CAACCTCTAG
1261GCTATGACAG GTCATCCCGC TCTTCCTATG TGGATGAGGA CTGTGACTCC CTGGCCCTCT
1321GTGATCCTCT GCAGAGGACA AATTGTGAAG TTGACAGCCT GCCTGAAAGC TGCTTCGACA
1381GCGGCTTGTC TACCTTGAGA GATCCCAATG AGTATGACTC AGAAGTGGAA TACAAGCACC
1441AGAGGGGATT TCAGAGGTCA CACGGGGTGC AGGAGAGCTT TGGAGGTGAT GCTTCAGACA
1501CAGATGTTCC TGACATAAGG GATGAAGAGA CATTTGGTTT AGAAGATGTG GCTTCCGTCT
1561TAGACTGGAA GCCCCAAGGG TCTGTTAGTG AAGGCAGCAT TGTTAGTTCA TCAAGAAAGC
1621CCATCTCAGC ACTCTCGCCC CAGACAGACC TGGTAGATGA CAACGCTAAA TCTTTTAGCT
1681CACAGAAGGC TTACAAGATT GTACTTGCTG GGGACGCTGC AGTGGGGAAG TCTAGTTTCC
1741TCATGAGACT TTGCAAGAAT GAATTTCGAG AAAATATAAG CGCCACCCTG GGAGTTGATT
1801TCCAAATGAA AACCCTCATT GTGGATGGAG AACGAACAGT TCTGCAGCTC TGGGATACAG
1861CTGGTCAGGA GAGATTCAGA AGTATTGCCA AGTCTTACTT CAGAAAGGCA GATGGTGTTT
1921TGCTGCTGTA TGATGTTACA TGTGAGAAAA GCTTTCTTAA CATACGAGAA TGGGTAGATA
1981TGATTGAGGA TGCAGCCCAT GAGACTGTTC CCATTATGCT GGTAGGAAAC AAGGCTGACA
2041TTCGTGACAC TGCTGCTACA GAGGGACAAA AATGTGTCCC AGGGCACTTT GGAGAGAAAC
2101TGGCCATGAC GTATGGGGCA TTATTCTGTG AAACAAGTGC CAAAGATGGT TCTAACATAG
2161TGGAGGCTGT TCTGCACCTT GCTCGAGAAG TGAAAAAGAG AACTGACAAG GATGACAGCA
2221GATCCATTAC CAATCTAACC GGGACCAATT CCAAAAAGTC ACCACAGATG AAGAATTGTT
2281GCAATGGCTT GCCAACTTTC TTGTACAAAG TTGGcattat aagaaagcat tgcttatcaa
2341tttgttgcaa cgaac

Download FASTA (ORF) (Full)