Construct: ORF ccsbBroadEn_05200

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003542.1_s300c1, BRDN0000389406
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMIM14 (201895)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 201895 SMIM14 small integral membrane pro... NM_001317896.2 100% 100%
2 human 201895 SMIM14 small integral membrane pro... NM_001317897.2 100% 100%
3 human 201895 SMIM14 small integral membrane pro... NM_174921.3 100% 100%
4 mouse 68552 Smim14 small integral membrane pro... NM_133697.3 82.4% 89.8% (many diffs)
5 mouse 68552 Smim14 small integral membrane pro... XM_006504083.2 82.4% 89.8% (many diffs)
6 mouse 68552 Smim14 small integral membrane pro... XM_006504084.3 82.4% 89.8% (many diffs)
7 mouse 68552 Smim14 small integral membrane pro... XM_006504085.3 82.4% 89.8% (many diffs)
8 mouse 68552 Smim14 small integral membrane pro... XM_006504086.3 82.4% 89.8% (many diffs)
9 mouse 68552 Smim14 small integral membrane pro... XM_006504087.3 82.4% 89.8% (many diffs)
10 mouse 68552 Smim14 small integral membrane pro... XM_006504088.3 82.4% 89.8% (many diffs)
11 mouse 68552 Smim14 small integral membrane pro... XM_006504089.2 82.4% 89.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
363
ORF length:
297
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC AGAAGGTGGA TTTGATCCCT GTGAATGTGT TTGCTCTCAT GAACATGCAA
121TGAGAAGACT GATCAATCTG TTACGGCAGT CCCAGTCCTA CTGCACAGAC ACAGAGTGTC
181TTCAGGAATT ACCGGGACCC TCTGGTGATA ATGGCATCAG TGTTACAATG ATCTTGGTAG
241CCTGGATGGT TATTGCATTG ATCTTGTTCT TACTGAGACC TCCTAATCTA AGAGGATCCA
301GCCTACCTGG AAAGCCAACC AGTCCTCATA ATGGACAAGA TCCACCAGCT CCTCCTGTGG
361ACTACCNAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
421gcaacgaac

Download FASTA (ORF) (Full)