Construct: ORF ccsbBroadEn_05644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010693.1_s300c1, BRDN0000455499
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAPCD1 (401251)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401251 | SAPCD1 | suppressor APC domain conta... | NM_001039651.1 | 100% | 100% | |
| 2 | human | 100532732 | MSH5-SAPCD1 | MSH5-SAPCD1 readthrough (NM... | NR_037846.1 | 13.1% | 1_3207del;3742_4070del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 603
- ORF length:
- 534
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGGGAGCCAG GGCTCTGGCG GGGTGCCCTT GGTGCAGGCT CCCTACACAG 121 TCCTGCTGCT GCCGCTGGGG ACAAGCCGCC AAGACCCAGG GGCCCAGAGC TTCTTCCTTT 181 GGCTACGCAG GATGCAGGCT CTGGAGAGAG AACAGGATGC CCTGTGGCAG GGTCTGGAGC 241 TGCTACAGCA TGGCCAGGCC TGGTTTGAAG ACCATCTGAG GGAGGCACAG CGACAGCAGC 301 TGCATCTAGG GGCCCTTGGT GAGAATTTTC TAACAGATTT ACACTCAGAG CCTGGTCGCC 361 CCCCGTTAGC CCAGATTCAA AAGGTGAACA TCTGTTTGCA GAATCTGATT CATGAGAAGT 421 TCTCCCCAAG TCCACTGAAC AAGGCTAGTT CCTGCACCAC CCAGGATTCA AAGGAAAGAC 481 GAAGGGAGCA GAACTTGTGG CAGCAACAGG AGTTGTCAAG GCAGCAGAAA GGAGTCACCC 541 AGCCAAAGGA GGAGATGGCT CAGCGGGGCT GCACCAAGGG GCCAAGAGGC CCTACCCGTG 601 TCTTGCCAAC TTTCTTGTAC AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt 661 gcaacgaac