Construct: ORF ccsbBroadEn_06237
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006012.1_s300c1, BRDN0000457320
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAA (2548)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2548 | GAA | glucosidase alpha, acid | NM_000152.5 | 99.9% | 100% | 183C>T;2571G>T |
| 2 | human | 2548 | GAA | glucosidase alpha, acid | NM_001079803.3 | 99.9% | 100% | 183C>T;2571G>T |
| 3 | human | 2548 | GAA | glucosidase alpha, acid | NM_001079804.3 | 99.9% | 100% | 183C>T;2571G>T |
| 4 | human | 2548 | GAA | glucosidase alpha, acid | XM_005257193.2 | 99.9% | 100% | 183C>T;2571G>T |
| 5 | human | 2548 | GAA | glucosidase alpha, acid | XM_005257194.4 | 99.9% | 100% | 183C>T;2571G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2922
- ORF length:
- 2856
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG AGTGAGGCAC CCGCCCTGCT CCCACCGGCT CCTGGCCGTC TGCGCCCTCG 121 TGTCCTTGGC AACCGCTGCA CTCCTGGGGC ACATCCTACT CCATGATTTC CTGCTGGTTC 181 CCCGAGAGCT GAGTGGCTCC TCCCCAGTCC TGGAGGAGAC TCACCCAGCT CACCAGCAGG 241 GAGCCAGTAG ACCAGGGCCC CGGGATGCCC AGGCACACCC CGGCCGTCCC AGAGCAGTGC 301 CCACACAGTG CGACGTCCCC CCCAACAGCC GCTTCGATTG CGCCCCTGAC AAGGCCATCA 361 CCCAGGAACA GTGCGAGGCC CGCGGCTGTT GCTACATCCC TGCAAAGCAG GGGCTGCAGG 421 GAGCCCAGAT GGGGCAGCCC TGGTGCTTCT TCCCACCCAG CTACCCCAGC TACAAGCTGG 481 AGAACCTGAG CTCCTCTGAA ATGGGCTACA CGGCCACCCT GACCCGTACC ACCCCCACCT 541 TCTTCCCCAA GGACATCCTG ACCCTGCGGC TGGACGTGAT GATGGAGACT GAGAACCGCC 601 TCCACTTCAC GATCAAAGAT CCAGCTAACA GGCGCTACGA GGTGCCCTTG GAGACCCCGC 661 ATGTCCACAG CCGGGCACCG TCCCCACTCT ACAGCGTGGA GTTCTCCGAG GAGCCCTTCG 721 GGGTGATCGT GCGCCGGCAG CTGGACGGCC GCGTGCTGCT GAACACGACG GTGGCGCCCC 781 TGTTCTTTGC GGACCAGTTC CTTCAGCTGT CCACCTCGCT GCCCTCGCAG TATATCACAG 841 GCCTCGCCGA GCACCTCAGT CCCCTGATGC TCAGCACCAG CTGGACCAGG ATCACCCTGT 901 GGAACCGGGA CCTTGCGCCC ACGCCCGGTG CGAACCTCTA CGGGTCTCAC CCTTTCTACC 961 TGGCGCTGGA GGACGGCGGG TCGGCACACG GGGTGTTCCT GCTAAACAGC AATGCCATGG 1021 ATGTGGTCCT GCAGCCGAGC CCTGCCCTTA GCTGGAGGTC GACAGGTGGG ATCCTGGATG 1081 TCTACATCTT CCTGGGCCCA GAGCCCAAGA GCGTGGTGCA GCAGTACCTG GACGTTGTGG 1141 GATACCCGTT CATGCCGCCA TACTGGGGCC TGGGCTTCCA CCTGTGCCGC TGGGGCTACT 1201 CCTCCACCGC TATCACCCGC CAGGTGGTGG AGAACATGAC CAGGGCCCAC TTCCCCCTGG 1261 ACGTCCAGTG GAACGACCTG GACTACATGG ACTCCCGGAG GGACTTCACG TTCAACAAGG 1321 ATGGCTTCCG GGACTTCCCG GCCATGGTGC AGGAGCTGCA CCAGGGCGGC CGGCGCTACA 1381 TGATGATCGT GGATCCTGCC ATCAGCAGCT CGGGCCCTGC CGGGAGCTAC AGGCCCTACG 1441 ACGAGGGTCT GCGGAGGGGG GTTTTCATCA CCAACGAGAC CGGCCAGCCG CTGATTGGGA 1501 AGGTATGGCC CGGGTCCACT GCCTTCCCCG ACTTCACCAA CCCCACAGCC CTGGCCTGGT 1561 GGGAGGACAT GGTGGCTGAG TTCCATGACC AGGTGCCCTT CGACGGCATG TGGATTGACA 1621 TGAACGAGCC TTCCAACTTC ATCAGGGGCT CTGAGGACGG CTGCCCCAAC AATGAGCTGG 1681 AGAACCCACC CTACGTGCCT GGGGTGGTTG GGGGGACCCT CCAGGCGGCC ACCATCTGTG 1741 CCTCCAGCCA CCAGTTTCTC TCCACACACT ACAACCTGCA CAACCTCTAC GGCCTGACCG 1801 AAGCCATCGC CTCCCACAGG GCGCTGGTGA AGGCTCGGGG GACACGCCCA TTTGTGATCT 1861 CCCGCTCGAC CTTTGCTGGC CACGGCCGAT ACGCCGGCCA CTGGACGGGG GACGTGTGGA 1921 GCTCCTGGGA GCAGCTCGCC TCCTCCGTGC CAGAAATCCT GCAGTTTAAC CTGCTGGGGG 1981 TGCCTCTGGT CGGGGCCGAC GTCTGCGGCT TCCTGGGCAA CACCTCAGAG GAGCTGTGTG 2041 TGCGCTGGAC CCAGCTGGGG GCCTTCTACC CCTTCATGCG GAACCACAAC AGCCTGCTCA 2101 GTCTGCCCCA GGAGCCGTAC AGCTTCAGCG AGCCGGCCCA GCAGGCCATG AGGAAGGCCC 2161 TCACCCTGCG CTACGCACTC CTCCCCCACC TCTACACACT GTTCCACCAG GCCCACGTCG 2221 CGGGGGAGAC CGTGGCCCGG CCCCTCTTCC TGGAGTTCCC CAAGGACTCT AGCACCTGGA 2281 CTGTGGACCA CCAGCTCCTG TGGGGGGAGG CCCTGCTCAT CACCCCAGTG CTCCAGGCCG 2341 GGAAGGCCGA AGTGACTGGC TACTTCCCCT TGGGCACATG GTACGACCTG CAGACGGTGC 2401 CAGTAGAGGC CCTTGGCAGC CTCCCACCCC CACCTGCAGC TCCCCGTGAG CCAGCCATCC 2461 ACAGCGAGGG GCAGTGGGTG ACGCTGCCGG CCCCCCTGGA CACCATCAAC GTCCACCTCC 2521 GGGCTGGGTA CATCATCCCC CTGCAGGGCC CTGGCCTCAC AACCACAGAG TCCCGCCAGC 2581 AGCCCATGGC CCTGGCTGTG GCCCTGACCA AGGGTGGGGA GGCCCGAGGG GAGCTTTTCT 2641 GGGACGATGG AGAGAGCCTG GAAGTGCTGG AGCGAGGGGC CTACACACAG GTCATCTTCC 2701 TGGCCAGGAA TAACACGATC GTGAATGAGC TGGTACGTGT GACCAGTGAG GGAGCTGGCC 2761 TGCAGCTGCA GAAGGTGACT GTCCTGGGCG TGGCCACGGC GCCCCAGCAG GTCCTCTCCA 2821 ACGGTGTCCC TGTCTCCAAC TTCACCTACA GCCCCGACAC CAAGGTCCTG GACATCTGTG 2881 TCTCGCTGTT GATGGGAGAG CAGTTTCTCG TCAGCTGGTG TTACCCAACT TTCTTGTACA 2941 AAGTTGGcat tataagaaag cattgcttat caatttgttg caacgaac