Construct: ORF ccsbBroadEn_06250

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017225.1_s300c1, BRDN0000395876
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GALK1 (2584)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2584 GALK1 galactokinase 1 NM_000154.2 99.8% 99.7% 189G>A;1123A>G
2 mouse 14635 Galk1 galactokinase 1 NM_016905.2 85.3% 87.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1242
ORF length:
1176
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TGCTTTGAGA CAGCCCCAGG TCGCGGAGCT GCTGGCCGAG GCCCGGCGAG
121CCTTCCGGGA GGAGTTCGGG GCCGAGCCCG AGCTGGCCGT GTCAGCGCCG GGCCGCGTCA
181ACCTCATCGG GGAACACACG GACTACAACC AGGGCCTGGT GCTGCCTATG GCTCTGGAGC
241TCATGACGGT GCTAGTGGGC AGCCCCCGCA AGGATGGGCT GGTGTCTCTC CTCACCACCT
301CTGAGGGTGC CGATGAGCCC CAGCGGCTGC AGTTTCCACT GCCCACAGCC CAGCGCTCGC
361TGGAGCCTGG GACTCCTCGG TGGGCCAACT ATGTCAAGGG AGTGATTCAG TACTACCCAG
421CTGCCCCCCT CCCTGGCTTC AGTGCAGTGG TGGTCAGCTC AGTGCCCCTG GGGGGTGGCC
481TGTCCAGCTC AGCATCCTTG GAAGTGGCCA CGTACACCTT CCTCCAGCAG CTCTGTCCAG
541ACTCGGGCAC AATAGCTGCC CGCGCCCAGG TGTGTCAGCA GGCCGAGCAC AGCTTCGCAG
601GGATGCCCTG TGGCATCATG GACCAGTTCA TCTCACTTAT GGGACAGAAA GGCCACGCGC
661TGCTCATTGA CTGCAGGTCC TTGGAGACCA GCCTGGTGCC ACTCTCGGAC CCCAAGCTGG
721CCGTGCTCAT CACCAACTCT AATGTCCGCC ACTCCCTGGC CTCCAGCGAG TACCCTGTGC
781GGCGGCGCCA ATGTGAAGAA GTGGCCCGGG CGCTGGGCAA GGAAAGCCTC CGGGAGGTAC
841AACTGGAAGA GCTAGAGGCT GCCAGGGACC TGGTGAGCAA AGAGGGCTTC CGGCGGGCCC
901GGCACGTGGT GGGGGAGATT CGGCGCACGG CCCAGGCAGC GGCCGCCCTG AGACGTGGCG
961ACTACAGAGC CTTTGGCCGC CTCATGGTGG AGAGCCACCG CTCACTCAGA GACGACTATG
1021AGGTGAGCTG CCCAGAGCTG GACCAGCTGG TGGAGGCTGC GCTTGCTGTG CCTGGGGTTT
1081ATGGCAGCCG CATGACGGGC GGTGGCTTCG GTGGCTGCAC GGTGACACTG CTGGAGGCCT
1141CCGCTGCTCC CCACGCCATG CGGCACATCC AGGAGCACTA CGGCGGGGCT GCCACCTTCT
1201ACCTCTCTCA AGCAGCCGAT GGAGCCAAGG TGCTGTGCTT GTGCCCAACT TTCTTGTACA
1261AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)