Construct: ORF ccsbBroadEn_06288

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005604.1_s300c1, BRDN0000389129
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GMDS (2762)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2762 GMDS GDP-mannose 4,6-dehydratase NM_001500.4 99.9% 100% 798T>C
2 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_011514500.1 91.5% 90.8% (many diffs)
3 human 2762 GMDS GDP-mannose 4,6-dehydratase NM_001253846.1 91.4% 90.8% (many diffs)
4 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_011514502.3 89.2% 88.7% (many diffs)
5 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_017010752.1 73.9% 67.7% (many diffs)
6 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_006715066.3 72.9% 68.4% (many diffs)
7 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_011514503.3 68.7% 56.2% (many diffs)
8 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_011514505.2 64.3% 56.2% (many diffs)
9 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_011514506.2 63.6% 56% (many diffs)
10 human 2762 GMDS GDP-mannose 4,6-dehydratase XR_001743349.2 61.9% (many diffs)
11 human 2762 GMDS GDP-mannose 4,6-dehydratase XM_011514507.2 59.6% 58.6% 640_641ins128;666_667ins322
12 human 2762 GMDS GDP-mannose 4,6-dehydratase XR_001743350.2 12.4% (many diffs)
13 mouse 218138 Gmds GDP-mannose 4, 6-dehydratase NM_146041.2 89.3% 97% (many diffs)
14 mouse 218138 Gmds GDP-mannose 4, 6-dehydratase XM_006516656.2 70.1% 75.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1182
ORF length:
1116
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC ACACGCACCG GCACGCTGCC CCAGCGCCCG GGGCTCCGGG GACGGCGAGA
121TGGGCAAGCC CAGGAACGTG GCGCTCATCA CCGGTATCAC AGGCCAGGAT GGTTCCTACC
181TGGCTGAGTT CCTGCTGGAG AAAGGCTATG AGGTCCATGG AATTGTACGG CGGTCCAGTT
241CATTTAATAC GGGTCGAATT GAGCATCTGT ATAAGAATCC CCAGGCTCAC ATTGAAGGAA
301ACATGAAGTT GCACTATGGC GATCTCACTG ACAGTACCTG CCTTGTGAAG ATCATTAATG
361AAGTAAAGCC CACAGAGATC TACAACCTTG GAGCCCAGAG CCACGTCAAA ATTTCCTTTG
421ACCTCGCTGA GTACACTGCG GACGTTGACG GAGTTGGCAC TCTACGACTT CTAGATGCAG
481TTAAGACTTG TGGCCTTATC AACTCTGTGA AGTTCTACCA AGCCTCAACA AGTGAACTTT
541ATGGGAAAGT GCAGGAAATA CCCCAGAAGG AGACCACCCC TTTCTATCCC CGGTCACCCT
601ATGGGGCAGC AAAACTCTAT GCCTATTGGA TTGTGGTGAA CTTCCGTGAG GCGTATAATC
661TCTTTGCAGT GAACGGCATT CTCTTCAATC ATGAGAGTCC CAGAAGAGGA GCTAATTTCG
721TTACTCGAAA AATTAGCCGG TCAGTAGCTA AGATTTACCT TGGACAACTG GAATGTTTCA
781GTTTGGGAAA TCTGGATGCC AAACGAGATT GGGGCCATGC CAAGGACTAT GTGGAGGCTA
841TGTGGTTGAT GTTGCAGAAT GACGAGCCGG AGGACTTCGT TATAGCTACT GGGGAGGTCC
901ATAGTGTCCG GGAATTTGTC GAGAAATCAT TCTTGCACAT TGGAAAAACC ATTGTGTGGG
961AAGGAAAGAA TGAAAATGAA GTGGGCAGAT GTAAAGAGAC CGGCAAAGTT CACGTGACTG
1021TGGATCTCAA GTACTACCGG CCAACTGAAG TGGACTTTCT GCAGGGCGAC TGCACCAAAG
1081CGAAACAGAA GCTGAACTGG AAGCCCCGGG TCGCTTTCGA TGAGCTGGTG AGGGAGATGG
1141TGCACGCCGA CGTGGAGCTC ATGAGGACAA ACCCCAATGC CTGCCCAACT TTCTTGTACA
1201AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)