Construct: ORF ccsbBroadEn_06459
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002494.1_s300c1, BRDN0000389044
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IRF4 (3662)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3662 | IRF4 | interferon regulatory factor 4 | NM_002460.4 | 99.9% | 100% | 421C>T |
| 2 | human | 3662 | IRF4 | interferon regulatory factor 4 | NM_001195286.2 | 99.7% | 99.7% | 421C>T;489_490insCAG |
| 3 | human | 3662 | IRF4 | interferon regulatory factor 4 | XM_006715090.2 | 91.9% | 92% | 421C>T;636_637ins108 |
| 4 | human | 3662 | IRF4 | interferon regulatory factor 4 | NR_046000.3 | 24.7% | (many diffs) | |
| 5 | mouse | 16364 | Irf4 | interferon regulatory factor 4 | NM_013674.2 | 87.5% | 92.2% | (many diffs) |
| 6 | mouse | 16364 | Irf4 | interferon regulatory factor 4 | NM_001347508.1 | 87.3% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1419
- ORF length:
- 1353
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA CCTGGAGGGC GGCGGCCGAG GCGGAGAGTT CGGCATGAGC GCGGTGAGCT 121 GCGGCAACGG GAAGCTCCGC CAGTGGCTGA TCGACCAGAT CGACAGCGGC AAGTACCCCG 181 GGCTGGTGTG GGAGAACGAG GAGAAGAGCA TCTTCCGCAT CCCCTGGAAG CACGCGGGCA 241 AGCAGGACTA CAACCGCGAG GAGGACGCCG CGCTCTTCAA GGCTTGGGCA CTGTTTAAAG 301 GAAAGTTCCG AGAAGGCATC GACAAGCCGG ACCCTCCCAC CTGGAAGACG CGCCTGCGGT 361 GCGCTTTGAA CAAGAGCAAT GACTTTGAGG AACTGGTTGA GCGGAGCCAG CTGGACATCT 421 CAGACCCGTA CAAAGTGTAC AGGATTGTTC CTGAGGGAGC CAAAAAAGGA GCCAAGCAGC 481 TCACCTTGGA GGACCCGCAG ATGTCCATGA GCCACCCCTA CACCATGACA ACGCCTTACC 541 CTTCGCTCCC AGCCCAGCAG GTTCACAACT ACATGATGCC ACCCCTCGAC CGAAGCTGGA 601 GGGACTACGT CCCGGATCAG CCACACCCGG AAATCCCGTA CCAATGTCCC ATGACGTTTG 661 GACCCCGCGG CCACCACTGG CAAGGCCCAG CTTGTGAAAA TGGTTGCCAG GTGACAGGAA 721 CCTTTTATGC TTGTGCCCCA CCTGAGTCCC AGGCTCCCGG AGTCCCCACA GAGCCAAGCA 781 TAAGGTCTGC CGAAGCCTTG GCGTTCTCAG ACTGCCGGCT GCACATCTGC CTGTACTACC 841 GGGAAATCCT CGTGAAGGAG CTGACCACGT CCAGCCCCGA GGGCTGCCGG ATCTCCCATG 901 GACATACGTA TGACGCCAGC AACCTGGACC AGGTCCTGTT CCCCTACCCA GAGGACAATG 961 GCCAGAGGAA AAACATTGAG AAGCTGCTGA GCCACCTGGA GAGGGGCGTG GTCCTCTGGA 1021 TGGCCCCCGA CGGGCTCTAT GCGAAAAGAC TGTGCCAGAG CAGGATCTAC TGGGACGGGC 1081 CCCTGGCGCT GTGCAACGAC CGGCCCAACA AACTGGAGAG AGACCAGACC TGCAAGCTCT 1141 TTGACACACA GCAGTTCTTG TCAGAGCTGC AAGCGTTTGC TCACCACGGC CGCTCCCTGC 1201 CAAGATTCCA GGTGACTCTA TGCTTTGGAG AGGAGTTTCC AGACCCTCAG AGGCAAAGAA 1261 AGCTCATCAC AGCTCACGTA GAACCTCTGC TAGCCAGACA ACTATATTAT TTTGCTCAAC 1321 AAAACAGTGG ACATTTCCTG AGGGGCTACG ATTTACCAGA ACACATCAGC AATCCAGAAG 1381 ATTACCACAG ATCTATCCGC CATTCCTCTA TTCAAGAATG CCCAACTTTC TTGTACAAAG 1441 Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac