Construct: ORF ccsbBroadEn_06463
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004914.1_s300c1, BRDN0000391371
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGAM (3684)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3684 | ITGAM | integrin subunit alpha M | NM_000632.4 | 99.9% | 99.8% | 1322T>C;3399C>T;3442G>N |
| 2 | human | 3684 | ITGAM | integrin subunit alpha M | NM_001145808.2 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 3684 | ITGAM | integrin subunit alpha M | XM_011545850.2 | 92.8% | 93% | (many diffs) |
| 4 | human | 3684 | ITGAM | integrin subunit alpha M | XR_950796.1 | 80.6% | (many diffs) | |
| 5 | human | 3684 | ITGAM | integrin subunit alpha M | XM_017023216.1 | 78.9% | 78.5% | (many diffs) |
| 6 | human | 3684 | ITGAM | integrin subunit alpha M | XM_011545851.2 | 53.8% | 53.4% | (many diffs) |
| 7 | human | 3684 | ITGAM | integrin subunit alpha M | XM_006721045.1 | 53.1% | 53.1% | 1322T>C;1839_1840ins1617 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3525
- ORF length:
- 3456
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCTCTCAGA GTCCTTCTGT TAACAGCCTT GACCTTATGT CATGGGTTCA 121 ACTTGGACAC TGAAAACGCA ATGACCTTCC AAGAGAACGC AAGGGGCTTC GGGCAGAGCG 181 TGGTCCAGCT TCAGGGATCC AGGGTGGTGG TTGGAGCCCC CCAGGAGATA GTGGCTGCCA 241 ACCAAAGGGG CAGCCTCTAC CAGTGCGACT ACAGCACAGG CTCATGCGAG CCCATCCGCC 301 TGCAGGTCCC CGTGGAGGCC GTGAACATGT CCCTGGGCCT GTCCCTGGCA GCCACCACCA 361 GCCCCCCTCA GCTGCTGGCC TGTGGTCCCA CCGTGCACCA GACTTGCAGT GAGAACACGT 421 ATGTGAAAGG GCTCTGCTTC CTGTTTGGAT CCAACCTACG GCAGCAGCCC CAGAAGTTCC 481 CAGAGGCCCT CCGAGGGTGT CCTCAAGAGG ATAGTGACAT TGCCTTCTTG ATTGATGGCT 541 CTGGTAGCAT CATCCCACAT GACTTTCGGC GGATGAAGGA GTTTGTCTCA ACTGTGATGG 601 AGCAATTAAA AAAGTCCAAA ACCTTGTTCT CTTTGATGCA GTACTCTGAA GAATTCCGGA 661 TTCACTTTAC CTTCAAAGAG TTCCAGAACA ACCCTAACCC AAGATCACTG GTGAAGCCAA 721 TAACGCAGCT GCTTGGGCGG ACACACACGG CCACGGGCAT CCGCAAAGTG GTACGAGAGC 781 TGTTTAACAT CACCAACGGA GCCCGAAAGA ATGCCTTTAA GATCCTAGTT GTCATCACGG 841 ATGGAGAAAA GTTTGGCGAT CCCTTGGGAT ATGAGGATGT CATCCCTGAG GCAGACAGAG 901 AGGGAGTCAT TCGCTACGTC ATTGGGGTGG GAGATGCCTT CCGCAGTGAG AAATCCCGCC 961 AAGAGCTTAA TACCATCGCA TCCAAGCCGC CTCGTGATCA CGTGTTCCAG GTGAATAACT 1021 TTGAGGCTCT GAAGACCATT CAGAACCAGC TTCGGGAGAA GATCTTTGCG ATCGAGGGTA 1081 CTCAGACAGG AAGTAGCAGC TCCTTTGAGC ATGAGATGTC TCAGGAAGGC TTCAGCGCTG 1141 CCATCACCTC TAATGGCCCC TTGCTGAGCA CTGTGGGGAG CTATGACTGG GCTGGTGGAG 1201 TCTTTCTATA TACATCAAAG GAGAAAAGCA CCTTCATCAA CATGACCAGA GTGGATTCAG 1261 ACATGAATGA TGCTTACTTG GGTTATGCTG CCGCCATCAT CTTACGGAAC CGGGTGCAAA 1321 GCCTGGTTCT GGGGGCACCT CGATATCAGC ACATCGGCCT GGTAGCGATG TTCAGGCAGA 1381 ACACTGGCAC GTGGGAGTCC AACGCTAATG TCAAGGGCAC CCAGATCGGC GCCTACTTCG 1441 GGGCCTCCCT CTGCTCCGTG GACGTGGACA GCAACGGCAG CACCGACCTG GTCCTCATCG 1501 GGGCCCCCCA TTACTACGAG CAGACCCGAG GGGGCCAGGT GTCCGTGTGC CCCTTGCCCA 1561 GGGGGAGGGC TCGGTGGCAG TGTGATGCTG TTCTCTACGG GGAGCAGGGC CAACCCTGGG 1621 GCCGCTTTGG GGCAGCCCTA ACAGTGCTGG GGGACGTAAA TGGGGACAAG CTGACGGACG 1681 TGGCCATTGG GGCCCCAGGA GAGGAGGACA ACCGGGGTGC TGTTTACCTG TTTCACGGAA 1741 CCTCAGGATC TGGCATCAGC CCCTCCCATA GCCAGCGGAT AGCAGGCTCC AAGCTCTCTC 1801 CCAGGCTCCA GTATTTTGGT CAGTCACTGA GTGGGGGCCA GGACCTCACA ATGGATGGAC 1861 TGGTAGACCT GACTGTAGGA GCCCAGGGGC ACGTGCTGCT GCTCAGGTCC CAGCCAGTAC 1921 TGAGAGTCAA GGCAATCATG GAGTTCAATC CCAGGGAAGT GGCAAGGAAT GTATTTGAGT 1981 GTAATGATCA GGTGGTGAAA GGCAAGGAAG CCGGAGAGGT CAGAGTCTGC CTCCATGTCC 2041 AGAAGAGCAC ACGGGATCGG CTAAGAGAAG GACAGATCCA GAGTGTTGTG ACTTATGACC 2101 TGGCTCTGGA CTCCGGCCGC CCACATTCCC GCGCCGTCTT CAATGAGACA AAGAACAGCA 2161 CACGCAGACA GACACAGGTC TTGGGGCTGA CCCAGACTTG TGAGACCCTG AAACTACAGT 2221 TGCCGAATTG CATCGAGGAC CCAGTGAGCC CCATTGTGCT GCGCCTGAAC TTCTCTCTGG 2281 TGGGAACGCC ATTGTCTGCT TTCGGGAACC TCCGGCCAGT GCTGGCGGAG GATGCTCAGA 2341 GACTCTTCAC AGCCTTGTTT CCCTTTGAGA AGAATTGTGG CAATGACAAC ATCTGCCAGG 2401 ATGACCTCAG CATCACCTTC AGTTTCATGA GCCTGGACTG CCTCGTGGTG GGTGGGCCCC 2461 GGGAGTTCAA CGTGACAGTG ACTGTGAGAA ATGATGGTGA GGACTCCTAC AGGACACAGG 2521 TCACCTTCTT CTTCCCGCTT GACCTGTCCT ACCGGAAGGT GTCCACGCTC CAGAACCAGC 2581 GCTCACAGCG ATCCTGGCGC CTGGCCTGTG AGTCTGCCTC CTCCACCGAA GTGTCTGGGG 2641 CCTTGAAGAG CACCAGCTGC AGCATAAACC ACCCCATCTT CCCGGAAAAC TCAGAGGTCA 2701 CCTTTAATAT CACGTTTGAT GTAGACTCTA AGGCTTCCCT TGGAAACAAA CTGCTCCTCA 2761 AGGCCAATGT GACCAGTGAG AACAACATGC CCAGAACCAA CAAAACCGAA TTCCAACTGG 2821 AGCTGCCGGT GAAATATGCT GTCTACATGG TGGTCACCAG CCATGGGGTC TCCACTAAAT 2881 ATCTCAACTT CACGGCCTCA GAGAATACCA GTCGGGTCAT GCAGCATCAA TATCAGGTCA 2941 GCAACCTGGG GCAGAGGAGC CTCCCCATCA GCCTGGTGTT CTTGGTGCCC GTCCGGCTGA 3001 ACCAGACTGT CATATGGGAC CGCCCCCAGG TCACCTTCTC CGAGAACCTC TCGAGTACGT 3061 GCCACACCAA GGAGCGCTTG CCCTCTCACT CCGACTTTCT GGCTGAGCTT CGGAAGGCCC 3121 CCGTGGTGAA CTGCTCCATC GCTGTCTGCC AGAGAATCCA GTGTGACATC CCGTTCTTTG 3181 GCATCCAGGA AGAATTCAAT GCTACCCTCA AAGGCAACCT CTCGTTTGAC TGGTACATCA 3241 AGACCTCGCA TAACCACCTC CTGATCGTGA GCACAGCTGA GATCTTGTTT AACGATTCCG 3301 TGTTCACCCT GCTGCCGGGA CAGGGGGCGT TTGTGAGGTC CCAGACGGAG ACCAAAGTGG 3361 AGCCGTTCGA GGTCCCCAAC CCCCTGCCGC TCATCGTGGG CAGCTCTGTC GGGGGACTGC 3421 TGCTCCTGGC CCTCATCACC GCCGCGCTGT ACAAGCTCGG CTTCTTTAAG CGGCAATACA 3481 AGGACATGAT GAGTGAAGGG GGTCCCCCGN GGGCCGAACC CCAGTTGCCA ACTTTCTTGT 3541 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c