Construct: ORF ccsbBroadEn_06525

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006663.1_s300c1, BRDN0000395897
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIPA (3988)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3988 LIPA lipase A, lysosomal acid type NM_000235.4 99.8% 99.4% 46A>C;85G>T
2 human 3988 LIPA lipase A, lysosomal acid type NM_001127605.3 99.8% 99.4% 46A>C;85G>T
3 human 3988 LIPA lipase A, lysosomal acid type XM_024448023.1 99.8% 99.4% 46A>C;85G>T
4 human 3988 LIPA lipase A, lysosomal acid type NM_001288979.1 70.9% 70.9% 0_1ins348
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1263
ORF length:
1197
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA AATGCGGTTC TTGGGGTTGG TGGTCTGTTT GGTTCTCTGG CCCCTGCATT
121CTGAGGGGTC TGGAGGGAAA CTGACAGCTT TGGATCCTGA AACAAACATG AATGTGAGTG
181AAATTATCTC TTACTGGGGA TTCCCTAGTG AGGAATACCT AGTTGAGACA GAAGATGGAT
241ATATTCTGTG CCTTAACCGA ATTCCTCATG GGAGGAAGAA CCATTCTGAC AAAGGTCCCA
301AACCAGTTGT CTTCCTGCAA CATGGCTTGC TGGCAGATTC TAGTAACTGG GTCACAAACC
361TTGCCAACAG CAGCCTGGGC TTCATTCTTG CTGATGCTGG TTTTGACGTG TGGATGGGCA
421ACAGCAGAGG AAATACCTGG TCTCGGAAAC ATAAGACACT CTCAGTTTCT CAGGATGAAT
481TCTGGGCTTT CAGTTATGAT GAGATGGCAA AATATGACCT ACCAGCTTCC ATTAACTTCA
541TTCTGAATAA AACTGGCCAA GAACAAGTGT ATTATGTGGG TCATTCTCAA GGCACCACTA
601TAGGTTTTAT AGCATTTTCA CAGATCCCTG AGCTGGCTAA AAGGATTAAA ATGTTTTTTG
661CCCTGGGTCC TGTGGCTTCC GTCGCCTTCT GTACTAGCCC TATGGCCAAA TTAGGACGAT
721TACCAGATCA TCTCATTAAG GACTTATTTG GAGACAAAGA ATTTCTTCCC CAGAGTGCGT
781TTTTGAAGTG GCTGGGTACC CACGTTTGCA CTCATGTCAT ACTGAAGGAG CTCTGTGGAA
841ATCTCTGTTT TCTTCTGTGT GGATTTAATG AGAGAAATTT AAATATGTCT AGAGTGGATG
901TATATACAAC ACATTCTCCT GCTGGAACTT CTGTGCAAAA CATGTTACAC TGGAGCCAGG
961CTGTTAAATT CCAAAAGTTT CAAGCCTTTG ACTGGGGAAG CAGTGCCAAG AATTATTTTC
1021ATTACAACCA GAGTTATCCT CCCACATACA ATGTGAAGGA CATGCTTGTG CCGACTGCAG
1081TCTGGAGCGG GGGTCACGAC TGGCTTGCAG ATGTCTACGA CGTCAATATC TTACTGACTC
1141AGATCACCAA CTTGGTGTTC CATGAGAGCA TTCCGGAATG GGAGCATCTT GACTTCATTT
1201GGGGCCTGGA TGCCCCTTGG AGGCTTTATA ATAAAATTAT TAATCTAATG AGGAAATATC
1261AGTGCCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
1321gcaacgaac

Download FASTA (ORF) (Full)