Construct: ORF ccsbBroadEn_06621

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012554.1_s300c1, BRDN0000455411
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NDUFA8 (4702)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4702 NDUFA8 NADH:ubiquinone oxidoreduct... NM_014222.3 99.6% 100% 126G>A;219G>A
2 human 4702 NDUFA8 NADH:ubiquinone oxidoreduct... NM_001318195.2 73.6% 73.8% 126G>A;219G>A;383_384delGGins134
3 mouse 68375 Ndufa8 NADH dehydrogenase (ubiquin... NM_026703.2 83.2% 86.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
582
ORF length:
516
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC GGGGATAGTG GAGCTGCCCA CTCTAGAGGA GCTGAAAGTA GATGAGGTGA
121AAATTAGTTC TGCTGTGCTT AAAGCTGCGG CCCATCACTA TGGAGCTCAA TGTGATAAGC
181CCAACAAGGA ATTTATGCTC TGCCGCTGGG AAGAGAAAGA TCCGAGGCGG TGTTTAGAGG
241AAGGCAAACT GGTCAACAAG TGTGCTTTGG ACTTCTTTAG GCAAATAAAA CGTCACTGTG
301CAGAGCCTTT TACAGAATAT TGGACTTGCA TTGATTATAC TGGCCAGCAG TTATTTCGTC
361ACTGTCGCAA ACAGCAGGCA AAGTTTGACG AGTGTGTGCT GGACAAACTG GGCTGGGTGC
421GGCCTGACCT GGGAGAACTG TCAAAGGTCA CCAAAGTGAA AACAGATCGA CCTTTACCGG
481AGAATCCCTA TCACTCAAGA CCAAGACCGG ATCCCAGCCC TGAGATCGAG GGAGATCTGC
541AGCCTGCCAC ACATGGCAGC CGCTTTTATT TCTGGACCAA GTACCCAACT TTCTTGTACA
601AAGTTGGcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)