Construct: ORF ccsbBroadEn_06658

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013905.1_s300c1, BRDN0000390959
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NT5E (4907)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4907 NT5E 5'-nucleotidase ecto NM_002526.4 99.8% 99.6% 1126A>G;1136T>C
2 human 4907 NT5E 5'-nucleotidase ecto NM_001204813.1 91.1% 90.9% 1126A>G;1136T>C;1209_1210ins150
3 mouse 23959 Nt5e 5' nucleotidase, ecto NM_011851.4 85.3% 86.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1788
ORF length:
1722
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTG TCCCCGAGCC GCGCGGGCGC CCGCGACGCT ACTCCTCGCC CTGGGCGCGG
121TGCTGTGGCC TGCGGCTGGC GCCTGGGAGC TTACGATTTT GCACACCAAC GACGTGCACA
181GCCGGCTGGA GCAGACCAGC GAGGACTCCA GCAAGTGCGT CAACGCCAGC CGCTGCATGG
241GTGGCGTGGC TCGGCTCTTC ACCAAGGTTC AGCAGATCCG CCGCGCCGAA CCCAACGTGC
301TGCTGCTGGA CGCCGGCGAC CAGTACCAGG GCACTATCTG GTTCACCGTG TACAAGGGCG
361CCGAGGTGGC GCACTTCATG AACGCCCTGC GCTACGATGC CATGGCACTG GGAAATCATG
421AATTTGATAA TGGTGTGGAA GGACTGATCG AGCCACTCCT CAAAGAGGCC AAATTTCCAA
481TTCTGAGTGC AAACATTAAA GCAAAGGGGC CACTAGCATC TCAAATATCA GGACTTTATT
541TGCCATATAA AGTTCTTCCT GTTGGTGATG AAGTTGTGGG AATCGTTGGA TACACTTCCA
601AAGAAACCCC TTTTCTCTCA AATCCAGGGA CAAATTTAGT GTTTGAAGAT GAAATCACTG
661CATTACAACC TGAAGTAGAT AAGTTAAAAA CTCTAAATGT GAACAAAATT ATTGCACTGG
721GACATTCGGG TTTTGAAATG GATAAACTCA TCGCTCAGAA AGTGAGGGGT GTGGACGTCG
781TGGTGGGAGG ACACTCCAAC ACATTTCTTT ACACAGGCAA TCCACCTTCC AAAGAGGTGC
841CTGCTGGGAA GTACCCATTC ATAGTCACTT CTGATGATGG GCGGAAGGTT CCTGTAGTCC
901AGGCCTATGC TTTTGGCAAA TACCTAGGCT ATCTGAAGAT CGAGTTTGAT GAAAGAGGAA
961ACGTCATCTC TTCCCATGGA AATCCCATTC TTCTAAACAG CAGCATTCCT GAAGATCCAA
1021GCATAAAAGC AGACATTAAC AAATGGAGGA TAAAATTGGA TAATTATTCT ACCCAGGAAT
1081TAGGGAAAAC AATTGTCTAT CTGGATGGCT CCTCTCAATC ATGCCGCTTT AGAGAATGCA
1141ACATGGGCAA CCTGATTTGT GATGCAATGA TTAACAACAA CCTGAGACAC GCGGATGAAA
1201CGTTCTGGAA CCACGTATCC ATGTGCATTT TAAATGGAGG TGGTATCCGG TCGCCCATTG
1261ATGAACGCAA CAATGGCACA ATTACCTGGG AGAACCTGGC TGCTGTATTG CCCTTTGGAG
1321GCACATTTGA CCTAGTCCAG TTAAAAGGTT CCACCCTGAA GAAGGCCTTT GAGCATAGCG
1381TGCACCGCTA CGGCCAGTCC ACTGGAGAGT TCCTGCAGGT GGGCGGAATC CATGTGGTGT
1441ATGATCTTTC CCGAAAACCT GGAGACAGAG TAGTCAAATT AGATGTTCTT TGCACCAAGT
1501GTCGAGTGCC CAGTTATGAC CCTCTCAAAA TGGACGAGGT ATATAAGGTG ATCCTCCCAA
1561ACTTCCTGGC CAATGGTGGA GATGGGTTCC AGATGATAAA AGATGAATTA TTAAGACATG
1621ACTCTGGTGA CCAAGATATC AACGTGGTTT CTACATATAT CTCCAAAATG AAAGTAATTT
1681ATCCAGCAGT TGAAGGTCGG ATCAAGTTTT CCACAGGAAG TCACTGCCAT GGAAGCTTTT
1741CTTTAATATT TCTTTCACTT TGGGCAGTGA TCTTTGTTTT ATACCAATAC CCAACTTTCT
1801TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)