Transcript: Human NM_001204813.1

Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
NT5E (4907)
Length:
3936
CDS:
557..2131

Additional Resources:

NCBI RefSeq record:
NM_001204813.1
NBCI Gene record:
NT5E (4907)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001204813.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048756 GCACTGGGAAATCATGAATTT pLKO.1 896 CDS 100% 13.200 18.480 N NT5E n/a
2 TRCN0000301140 GCACTGGGAAATCATGAATTT pLKO_005 896 CDS 100% 13.200 18.480 N NT5E n/a
3 TRCN0000359700 GCTATCTGAAGATCGAGTTTG pLKO_005 1419 CDS 100% 10.800 15.120 N NT5E n/a
4 TRCN0000048755 CCCATTGATGAACGCAACAAT pLKO.1 1745 CDS 100% 5.625 7.875 N NT5E n/a
5 TRCN0000301051 CCCATTGATGAACGCAACAAT pLKO_005 1745 CDS 100% 5.625 7.875 N NT5E n/a
6 TRCN0000359770 TAAGTTTACGTGTCCAAATTT pLKO_005 2364 3UTR 100% 15.000 10.500 N NT5E n/a
7 TRCN0000048757 CAGGACTTTATTTGCCATATA pLKO.1 1020 CDS 100% 13.200 9.240 N NT5E n/a
8 TRCN0000323361 TGATTTGTGATGCAATGATTA pLKO_005 1644 CDS 100% 13.200 9.240 N NT5E n/a
9 TRCN0000359702 AGTGTCGAGTGCCCAGTTATG pLKO_005 1839 CDS 100% 10.800 7.560 N NT5E n/a
10 TRCN0000048754 CCTCTCAATCATGCCGCTTTA pLKO.1 1602 CDS 100% 10.800 7.560 N NT5E n/a
11 TRCN0000048753 CCCAGTTATGACCCTCTCAAA pLKO.1 1850 CDS 100% 4.950 3.465 N NT5E n/a
12 TRCN0000323281 ATTAACATAGGGCCCTATAAG pLKO_005 2326 3UTR 100% 13.200 7.920 N NT5E n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001204813.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06658 pDONR223 100% 91.1% 90.9% None 1126A>G;1136T>C;1209_1210ins150 n/a
2 ccsbBroad304_06658 pLX_304 0% 91.1% 90.9% V5 1126A>G;1136T>C;1209_1210ins150 n/a
3 TRCN0000469166 AAATAATGTCAGCCTCCGGTTTAA pLX_317 24.8% 91.1% 90.9% V5 1126A>G;1136T>C;1209_1210ins150 n/a
4 ccsbBroadEn_11004 pDONR223 100% 49.7% 47.5% None (many diffs) n/a
5 ccsbBroad304_11004 pLX_304 0% 49.7% 47.5% V5 (many diffs) n/a
6 TRCN0000473589 TTACAAAGCAGGGAAGAGGAGGGG pLX_317 34.6% 49.7% 47.5% V5 (many diffs) n/a
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