Construct: ORF ccsbBroadEn_06738

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006259.1_s300c1, BRDN0000385936
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLEK (5341)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5341 PLEK pleckstrin NM_002664.3 99.9% 99.7% 1019G>A
2 mouse 56193 Plek pleckstrin NM_019549.2 88.5% 92% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1116
ORF length:
1050
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA ACCAAAGCGG ATCAGAGAGG GCTACCTTGT GAAGAAGGGG AGCGTGTTCA
121ATACGTGGAA ACCCATGTGG GTTGTATTGT TAGAAGATGG AATTGAATTC TATAAGAAGA
181AAAGTGACAA CAGCCCCAAA GGAATGATCC CGCTGAAAGG GAGCACTCTG ACTAGCCCTT
241GTCAAGACTT TGGCAAAAGG ATGTTTGTGT TTAAGATCAC TACGACCAAA CAGCAGGACC
301ACTTCTTCCA GGCAGCCTTC CTGGAGGAGA GAGATGCCTG GGTTCGGGAT ATCAAGAAGG
361CCATTAAATG CATTGAAGGA GGCCAGAAAT TTGCCAGGAA ATCTACCAGG AGGTCCATTC
421GACTGCCAGA AACCATTGAC TTAGGTGCCT TATATTTGTC CATGAAAGAC ACTGAAAAAG
481GAATAAAAGA ACTGAATCTA GAGAAGGACA AGAAGATTTT TAATCACTGC TTCACAGGTA
541ACTGCGTCAT TGATTGGCTG GTATCCAACC AGTCTGTTAG GAATCGCCAG GAAGGCCTCA
601TGATTGCTTC ATCGCTGCTC AATGAGGGGT ATCTGCAGCC TGCTGGAGAC ATGTCCAAGA
661GTGCAGTGGA TGGAACTGCT GAAAACCCTT TCCTGGACAA CCCTGATGCC TTCTACTACT
721TTCCAGACAG TGGGTTCTTC TGTGAAGAGA ATTCCAGTGA TGATGATGTG ATTCTGAAAG
781AAGAATTCAG AGGGGTCATT ATCAAGCAGG GATGTTTACT GAAGCAGGGG CATAGAAGGA
841AAAACTGGAA AGTGAGGAAG TTCATCTTGA GAGAAGACCC TGCCTACCTG CACTACTATG
901ACCCTGCTGG GGCAGAAGAT CCCCTGGGAG CAATTCACTT GAGAGGCTGT GTGGTGACTT
961CAGTGGAGAG CAACTCAAAT GGCAGGAAGA GTGAGGAAGA GAACCTTTTT GAGATCATCA
1021CAGCAGATGA AGTGCACTAT TTCTTGCAAG CAGCCACCCC CAAGGAGCGC ACAGAGTGGA
1081TCAAAGCCAT CCAGATGGCC TCCCGAACTG GGAAGTACCC AACTTTCTTG GACAAAGTtg
1141gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)