Construct: ORF ccsbBroadEn_06947
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006264.1_s300c1, BRDN0000386412
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHMT2 (6472)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_005412.6 | 99.9% | 100% | 813G>A |
| 2 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166356.2 | 97.9% | 98% | 594_595ins30;783G>A |
| 3 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166357.1 | 95.7% | 95.8% | 0_1ins63;750G>A |
| 4 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166358.2 | 95.7% | 95.8% | 0_1ins63;750G>A |
| 5 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NM_001166359.1 | 95.7% | 95.8% | 0_1ins63;750G>A |
| 6 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538675.3 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 7 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538676.3 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 8 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538677.3 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 9 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | XM_011538678.2 | 77.5% | 62.4% | 0_1ins316;279_306del;525G>A |
| 10 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_029417.2 | 66.3% | (many diffs) | |
| 11 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_029416.2 | 65.9% | (many diffs) | |
| 12 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_029415.1 | 65.2% | (many diffs) | |
| 13 | human | 6472 | SHMT2 | serine hydroxymethyltransfe... | NR_048562.1 | 57.9% | (many diffs) | |
| 14 | mouse | 108037 | Shmt2 | serine hydroxymethyltransfe... | NM_028230.4 | 87.9% | 93.8% | (many diffs) |
| 15 | mouse | 108037 | Shmt2 | serine hydroxymethyltransfe... | NM_001252316.1 | 87.3% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 1579
- ORF length:
- 1512
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACANAAAAA 61 GTTGGCATGC TGTACTTCTC TTTGTTTTGG GCGGCTCGGC CTCTGCAGAG ATGTGGGCAG 121 CTGGTCAGGA TGGCCATTCG GGCTCAGCAC AGCAACGCAG CCCAGACTCA GACTGGGGAA 181 GCAAACAGGG GCTGGACAGG CCAGGAGAGC CTGTCGGACA GTGATCCTGA GATGTGGGAG 241 TTGCTGCAGA GGGAGAAGGA CAGGCAGTGT CGTGGCCTGG AGCTCATTGC CTCAGAGAAC 301 TTCTGCAGCC GAGCTGCGCT GGAGGCCCTG GGGTCCTGTC TGAACAACAA GTACTCGGAG 361 GGTTATCCTG GCAAGAGATA CTATGGGGGA GCAGAGGTGG TGGATGAAAT TGAGCTGCTG 421 TGCCAGCGCC GGGCCTTGGA AGCCTTTGAC CTGGATCCTG CACAGTGGGG AGTCAATGTC 481 CAGCCCTACT CCGGGTCCCC AGCCAACCTG GCCGTCTACA CAGCCCTTCT GCAACCTCAC 541 GACCGGATCA TGGGGCTGGA CCTGCCCGAT GGGGGCCATC TCACCCACGG CTACATGTCT 601 GACGTCAAGC GGATATCAGC CACGTCCATC TTCTTCGAGT CTATGCCCTA TAAGCTCAAC 661 CCCAAAACTG GCCTCATTGA CTACAACCAG CTGGCACTGA CTGCTCGACT TTTCCGGCCA 721 CGGCTCATCA TAGCTGGCAC CAGCGCCTAT GCTCGCCTCA TTGACTACGC CCGCATGAGA 781 GAGGTGTGTG ATGAAGTCAA AGCACACCTG CTGGCAGACA TGGCCCACAT CAGTGGCCTG 841 GTGGCTGCCA AGGTGATTCC CTCGCCTTTC AAGCACGCAG ACATCGTCAC CACCACTACT 901 CACAAGACTC TTCGAGGGGC CAGGTCAGGG CTCATCTTCT ACCGGAAAGG GGTGAAGGCT 961 GTGGACCCCA AGACTGGCCG GGAGATCCCT TACACATTTG AGGACCGAAT CAACTTTGCC 1021 GTGTTCCCAT CCCTGCAGGG GGGCCCCCAC AATCATGCCA TTGCTGCAGT AGCTGTGGCC 1081 CTAAAGCAGG CCTGCACCCC CATGTTCCGG GAGTACTCCC TGCAGGTTCT GAAGAATGCT 1141 CGGGCCATGG CAGATGCCCT GCTAGAGCGA GGCTACTCAC TGGTATCAGG TGGTACTGAC 1201 AACCACCTGG TGCTGGTGGA CCTGCGGCCC AAGGGCCTGG ATGGAGCTCG GGCTGAGCGG 1261 GTGCTAGAGC TTGTATCCAT CACTGCCAAC AAGAACACCT GTCCTGGAGA CCGAAGTGCC 1321 ATCACACCGG GCGGCCTGCG GCTTGGGGCC CCAGCCTTAA CTTCTCGACA GTTCCGTGAG 1381 GATGACTTCC GGAGAGTTGT GGACTTTATA GATGAAGGGG TCAACATTGG CTTAGAGGTG 1441 AAGAGCAAGA CTGCCAAGCT CCAGGATTTC AAATCCTTCC TGCTTAAGGA CTCAGAAACA 1501 AGTCAGCGTC TGGCCAACCT CAGGCAACGG GTGGAGCAGT TTGCCAGGGC CTTCCCCATG 1561 CCTGGTTTTG ATGAGCATTG CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat 1621 tgcttatcaa tttgttgcaa cgaac