Construct: ORF ccsbBroadEn_06993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005235.1_s300c1, BRDN0000386006
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRD5A1 (6715)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001047.4 | 99.8% | 100% | 90C>G |
| 2 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001324322.2 | 75.8% | 48.4% | 1_26del;116C>G;318_319ins167 |
| 3 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001324323.2 | 67.6% | 63.7% | (many diffs) |
| 4 | human | 6719 | SRD5A1P1 | steroid 5 alpha-reductase 1... | NR_028597.1 | 34.3% | (many diffs) | |
| 5 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NR_136739.2 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 844
- ORF length:
- 777
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACANAAAAA 61 GTTGGCATGG CAACGGCGAC GGGGGTGGCG GAGGAGCGCC TGCTGGCCGC GCTCGCCTAC 121 CTGCAGTGCG CCGTGGGCTG CGCGGTCTTC GCGCGGAATC GTCAGACGAA CTCAGTGTAC 181 GGCCGCCACG CGCTGCCCAG CCACAGGCTC CGAGTGCCGG CGCGGGCCGC CTGGGTGGTG 241 CAGGAGCTGC CCTCGCTGGC CCTGCCGCTC TACCAGTACG CCAGCGAGTC CGCCCCGCGT 301 CTCCGCAGCG CGCCCAACTG CATCCTCCTG GCCATGTTCC TCGTCCACTA CGGGCATCGG 361 TGCTTAATTT ACCCATTTCT GATGCGAGGA GGAAAGCCTA TGCCACTGTT GGCGTGTACA 421 ATGGCGATTA TGTTCTGTAC CTGTAACGGC TATTTGCAAA GCAGATACTT GAGCCATTGT 481 GCAGTGTATG CTGATGACTG GGTAACAGAT CCCCGTTTTC TAATAGGTTT TGGCTTGTGG 541 TTAACGGGCA TGTTGATAAA CATCCATTCA GATCATATCC TAAGGAATCT CAGAAAACCA 601 GGAGATACTG GATACAAAAT ACCAAGGGGA GGCTTATTTG AATACGTAAC TGCAGCCAAC 661 TATTTTGGAG AAATCATGGA GTGGTGTGGC TATGCCCTGG CCAGCTGGTC TGTCCAAGGC 721 GCGGCTTTTG CTTTCTTCAC GTTTTGTTTT TTATCTGGTA GAGCAAAAGA GCATCATGAG 781 TGGTACCTCC GGAAATTTGA AGAGTATCCA AAGTTCAGAA AAATTATAAT TCCATTTTTG 841 TTTTACCCAA CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt 901 tgcaacgaac