Construct: ORF ccsbBroadEn_06993

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005235.1_s300c1, BRDN0000386006
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRD5A1 (6715)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NM_001047.4 99.8% 100% 90C>G
2 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NM_001324322.2 75.8% 48.4% 1_26del;116C>G;318_319ins167
3 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NM_001324323.2 67.6% 63.7% (many diffs)
4 human 6719 SRD5A1P1 steroid 5 alpha-reductase 1... NR_028597.1 34.3% (many diffs)
5 human 6715 SRD5A1 steroid 5 alpha-reductase 1 NR_136739.2 10.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
67
ORF end:
844
ORF length:
777
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACANAAAAA
61GTTGGCATGG CAACGGCGAC GGGGGTGGCG GAGGAGCGCC TGCTGGCCGC GCTCGCCTAC
121CTGCAGTGCG CCGTGGGCTG CGCGGTCTTC GCGCGGAATC GTCAGACGAA CTCAGTGTAC
181GGCCGCCACG CGCTGCCCAG CCACAGGCTC CGAGTGCCGG CGCGGGCCGC CTGGGTGGTG
241CAGGAGCTGC CCTCGCTGGC CCTGCCGCTC TACCAGTACG CCAGCGAGTC CGCCCCGCGT
301CTCCGCAGCG CGCCCAACTG CATCCTCCTG GCCATGTTCC TCGTCCACTA CGGGCATCGG
361TGCTTAATTT ACCCATTTCT GATGCGAGGA GGAAAGCCTA TGCCACTGTT GGCGTGTACA
421ATGGCGATTA TGTTCTGTAC CTGTAACGGC TATTTGCAAA GCAGATACTT GAGCCATTGT
481GCAGTGTATG CTGATGACTG GGTAACAGAT CCCCGTTTTC TAATAGGTTT TGGCTTGTGG
541TTAACGGGCA TGTTGATAAA CATCCATTCA GATCATATCC TAAGGAATCT CAGAAAACCA
601GGAGATACTG GATACAAAAT ACCAAGGGGA GGCTTATTTG AATACGTAAC TGCAGCCAAC
661TATTTTGGAG AAATCATGGA GTGGTGTGGC TATGCCCTGG CCAGCTGGTC TGTCCAAGGC
721GCGGCTTTTG CTTTCTTCAC GTTTTGTTTT TTATCTGGTA GAGCAAAAGA GCATCATGAG
781TGGTACCTCC GGAAATTTGA AGAGTATCCA AAGTTCAGAA AAATTATAAT TCCATTTTTG
841TTTTACCCAA CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt
901tgcaacgaac

Download FASTA (ORF) (Full)

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