Transcript: Human NR_136739.2

Homo sapiens steroid 5 alpha-reductase 1 (SRD5A1), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
SRD5A1 (6715)
Length:
7225
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_136739.2
NBCI Gene record:
SRD5A1 (6715)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_136739.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230329 GGGTAATAACTGCTGATATTT pLKO_005 1336 3UTR 100% 15.000 9.000 N SRD5A1 n/a
2 TRCN0000230326 CTACGGGCATCGGTGCTTAAT pLKO_005 419 3UTR 100% 13.200 7.920 N SRD5A1 n/a
3 TRCN0000230328 CTGTACCTGTAACGGCTATTT pLKO_005 506 3UTR 100% 13.200 7.920 N SRD5A1 n/a
4 TRCN0000230327 GCGTGTACAATGGCGATTATG pLKO_005 483 3UTR 100% 13.200 7.920 N SRD5A1 n/a
5 TRCN0000026585 CCAGGAGATACTGGATACAAA pLKO.1 859 3UTR 100% 5.625 3.375 N SRD5A1 n/a
6 TRCN0000026562 CGGCTATTTGCAAAGCAGATA pLKO.1 518 3UTR 100% 4.950 2.970 N SRD5A1 n/a
7 TRCN0000026546 GAGGCTTATTTGAATACGTAA pLKO.1 890 3UTR 100% 4.950 2.970 N SRD5A1 n/a
8 TRCN0000218975 CCATTCAGATCATATCCTAAG pLKO_005 825 3UTR 100% 6.000 3.000 Y SRD5A1 n/a
9 TRCN0000026523 CCTCCGGAAATTTGAAGAGTA pLKO.1 1047 3UTR 100% 4.950 2.475 Y SRD5A1 n/a
10 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 5334 3UTR 100% 4.950 2.475 Y LOC387873 n/a
11 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 5298 3UTR 100% 4.950 2.475 Y n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_136739.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06993 pDONR223 100% 10.7% None (many diffs) n/a
2 ccsbBroad304_06993 pLX_304 0% 10.7% V5 (many diffs) n/a
3 TRCN0000473518 TCCTACTTATTAACTTCATAGTAA pLX_317 54% 10.7% V5 (many diffs) n/a
4 TRCN0000491270 GCCGGGCCCCTCGCAAGAATAGTA pLX_317 30.6% 10.7% V5 (many diffs) n/a
5 TRCN0000488266 GAAGAGTGTCCTCTGGCGGATACA pLX_317 34.4% 10.7% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_10792 pDONR223 100% 3% None (many diffs) n/a
7 ccsbBroad304_10792 pLX_304 0% 3% V5 (many diffs) n/a
8 TRCN0000472287 GCCTGGAGAGCTTTCCTCGTCACG pLX_317 100% 3% V5 (many diffs) n/a
9 ccsbBroadEn_11616 pDONR223 100% 2.3% None (many diffs) n/a
10 ccsbBroad304_11616 pLX_304 0% 2.3% V5 (many diffs) n/a
11 TRCN0000467678 CCTCCCCTCACACCTCGTCAAAAC pLX_317 100% 2.3% V5 (many diffs) n/a
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