Construct: ORF ccsbBroadEn_07065

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012006.1_s300c1, BRDN0000457377
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
THBS2 (7058)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7058 THBS2 thrombospondin 2 NM_003247.3 99.9% 99.9% 5T>C
2 mouse 21826 Thbs2 thrombospondin 2 NM_011581.3 83.8% 88.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3585
ORF length:
3516
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCCTGGAGG CTGGTCCTGC TGGCTCTGTG GGTGTGGCCC AGCACGCAAG
121CTGGTCACCA GGACAAAGAC ACGACCTTCG ACCTTTTCAG TATCAGCAAC ATCAACCGCA
181AGACCATTGG CGCCAAGCAG TTCCGCGGGC CCGACCCCGG CGTGCCGGCT TACCGCTTCG
241TGCGCTTTGA CTACATCCCA CCGGTGAACG CAGATGACCT CAGCAAGATC ACCAAGATCA
301TGCGGCAGAA GGAGGGCTTC TTCCTCACGG CCCAGCTCAA GCAGGACGGC AAGTCCAGGG
361GCACGCTGTT GGCTCTGGAG GGCCCCGGTC TCTCCCAGAG GCAGTTCGAG ATCGTCTCCA
421ACGGCCCCGC GGACACGCTG GATCTCACCT ACTGGATTGA CGGCACCCGG CATGTGGTCT
481CCCTGGAGGA CGTCGGCCTG GCTGACTCGC AGTGGAAGAA CGTCACCGTG CAGGTGGCTG
541GCGAGACCTA CAGCTTGCAC GTGGGCTGCG ACCTCATAGA CAGCTTCGCT CTGGACGAGC
601CCTTCTACGA GCACCTGCAG GCGGAAAAGA GCCGGATGTA CGTGGCCAAA GGCTCTGCCA
661GAGAGAGTCA CTTCAGGGGT TTGCTTCAGA ACGTCCACCT AGTGTTTGAA AACTCTGTGG
721AAGATATTCT AAGCAAGAAG GGTTGCCAGC AAGGCCAGGG AGCTGAGATC AACGCCATCA
781GTGAGAACAC AGAGACGCTG CGCCTGGGTC CGCATGTCAC CACCGAGTAC GTGGGCCCCA
841GCTCGGAGAG GAGGCCCGAG GTGTGCGAAC GCTCGTGCGA GGAGCTGGGA AACATGGTCC
901AGGAGCTCTC GGGGCTCCAC GTCCTCGTGA ACCAGCTCAG CGAGAACCTC AAGAGAGTGT
961CGAATGATAA CCAGTTTCTC TGGGAGCTCA TTGGTGGCCC TCCTAAGACA AGGAACATGT
1021CAGCTTGCTG GCAGGATGGC CGGTTCTTTG CGGAAAATGA AACGTGGGTG GTGGACAGCT
1081GCACCACGTG TACCTGCAAG AAATTTAAAA CCATTTGCCA CCAAATCACC TGCCCGCCTG
1141CAACCTGCGC CAGTCCATCC TTTGTGGAAG GCGAATGCTG CCCTTCCTGC CTCCACTCGG
1201TGGACGGTGA GGAGGGCTGG TCTCCGTGGG CAGAGTGGAC CCAGTGCTCC GTGACGTGTG
1261GCTCTGGGAC CCAGCAGAGA GGCCGGTCCT GTGACGTCAC CAGCAACACC TGCTTGGGGC
1321CCTCCATCCA GACACGGGCT TGCAGTCTGA GCAAGTGTGA CACCCGCATC CGGCAGGACG
1381GCGGCTGGAG CCACTGGTCA CCTTGGTCTT CATGCTCTGT GACCTGTGGA GTTGGCAATA
1441TCACACGCAT CCGTCTCTGC AACTCCCCAG TGCCCCAGAT GGGGGGCAAG AATTGCAAAG
1501GGAGTGGCCG GGAGACCAAA GCCTGCCAGG GCGCCCCATG CCCAATCGAT GGCCGCTGGA
1561GCCCCTGGTC CCCGTGGTCG GCCTGCACTG TCACCTGTGC CGGTGGGATC CGGGAGCGCA
1621CCCGGGTCTG CAACAGCCCT GAGCCTCAGT ACGGAGGGAA GGCCTGCGTG GGGGATGTGC
1681AGGAGCGTCA GATGTGCAAC AAGAGGAGCT GCCCCGTGGA TGGCTGTTTA TCCAACCCCT
1741GCTTCCCGGG AGCCCAGTGC AGCAGCTTCC CCGATGGGTC CTGGTCATGC GGCTCCTGCC
1801CTGTGGGCTT CTTGGGCAAT GGCACCCACT GTGAGGACCT GGACGAGTGT GCCCTGGTCC
1861CCGACATCTG CTTCTCCACC AGCAAGGTGC CTCGCTGTGT CAACACTCAG CCTGGCTTCC
1921ACTGCCTGCC CTGCCCGCCC CGATACAGAG GGAACCAGCC CGTCGGGGTC GGCCTGGAAG
1981CAGCCAAGAC GGAAAAGCAA GTGTGTGAGC CCGAAAACCC ATGCAAGGAC AAGACACACA
2041ACTGCCACAA GCACGCGGAG TGCATCTACC TGGGCCACTT CAGCGACCCC ATGTACAAGT
2101GCGAGTGCCA GACAGGCTAC GCGGGCGACG GGCTCATCTG CGGGGAGGAC TCGGACCTGG
2161ACGGCTGGCC CAACCTCAAT CTGGTCTGCG CCACCAACGC CACCTACCAC TGCATCAAGG
2221ATAACTGCCC CCATCTGCCA AATTCTGGGC AGGAAGACTT TGACAAGGAC GGGATTGGCG
2281ATGCCTGTGA TGATGACGAT GACAATGACG GTGTGACCGA TGAGAAGGAC AACTGCCAGC
2341TCCTCTTCAA TCCCCGCCAG GCTGACTATG ACAAGGATGA GGTTGGGGAC CGCTGTGACA
2401ACTGCCCTTA CGTGCACAAC CCTGCCCAGA TCGACACAGA CAACAATGGA GAGGGTGACG
2461CCTGCTCCGT GGACATTGAT GGGGACGATG TCTTCAATGA ACGAGACAAT TGTCCCTACG
2521TCTACAACAC TGACCAGAGG GACACGGATG GTGACGGTGT GGGGGATCAC TGTGACAACT
2581GCCCCCTGGT GCACAACCCT GACCAGACCG ACGTGGACAA TGACCTTGTT GGGGACCAGT
2641GTGACAACAA CGAGGACATA GATGACGACG GCCACCAGAA CAACCAGGAC AACTGCCCCT
2701ACATCTCCAA CGCCAACCAG GCTGACCATG ACAGAGACGG CCAGGGCGAC GCCTGTGACC
2761CTGATGATGA CAACGATGGC GTCCCCGATG ACAGGGACAA CTGCCGGCTT GTGTTCAACC
2821CAGACCAGGA GGACTTGGAC GGTGATGGAC GGGGTGATAT TTGTAAAGAT GATTTTGACA
2881ATGACAACAT CCCAGATATT GATGATGTGT GTCCTGAAAA CAATGCCATC AGTGAGACAG
2941ACTTCAGGAA CTTCCAGATG GTCCCCTTGG ATCCCAAAGG GACCACCCAA ATTGATCCCA
3001ACTGGGTCAT TCGCCATCAA GGCAAGGAGC TGGTTCAGAC AGCCAACTCG GACCCCGGCA
3061TCGCTGTAGG TTTTGACGAG TTTGGGTCTG TGGACTTCAG TGGCACATTC TACGTAAACA
3121CTGACCGGGA CGACGACTAT GCCGGCTTCG TCTTTGGTTA CCAGTCAAGC AGCCGCTTCT
3181ATGTGGTGAT GTGGAAGCAG GTGACGCAGA CCTACTGGGA GGACCAGCCC ACGCGGGCCT
3241ATGGCTACTC CGGCGTGTCC CTCAAGGTGG TGAACTCCAC CACGGGGACG GGCGAGCACC
3301TGAGGAACGC GCTGTGGCAC ACGGGGAACA CGCCGGGGCA GGTGCGAACC TTATGGCACG
3361ACCCCAGGAA CATTGGCTGG AAGGACTACA CGGCCTATAG GTGGCACCTG ACTCACAGGC
3421CCAAGACTGG CTACATCAGA GTCTTAGTGC ATGAAGGAAA ACAGGTCATG GCAGACTCAG
3481GACCTATCTA TGACCAAACC TACGCTGGCG GGCGGCTGGG TCTATTTGTC TTCTCTCAAG
3541AAATGGTCTA TTTCTCAGAC CTCAAGTACG AATGCAGAGA TATTTTGCCA ACTTTCTTGT
3601ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)