Construct: ORF ccsbBroadEn_07104

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017821.1_s300c1, BRDN0000385960
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TSHR (7253)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7253 TSHR thyroid stimulating hormone... NM_001018036.3 99.7% 99.6% 696A>G;742C>A
2 human 7253 TSHR thyroid stimulating hormone... XM_005268039.1 99.7% 99.6% 696A>G;742C>A
3 human 7253 TSHR thyroid stimulating hormone... NM_001142626.3 92% 91.9% 693_755del;759A>G;805C>A
4 human 7253 TSHR thyroid stimulating hormone... XM_006720245.1 92% 91.9% 693_755del;759A>G;805C>A
5 human 7253 TSHR thyroid stimulating hormone... NM_000369.3 32.4% 30.1% (many diffs)
6 human 7253 TSHR thyroid stimulating hormone... XM_005268037.4 32.4% 30.1% (many diffs)
7 human 7253 TSHR thyroid stimulating hormone... XM_011537119.2 19.9% 16.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
825
ORF length:
759
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG GCCGGCGGAC TTGCTGCAGC TGGTGCTGCT GCTCGACCTG CCCAGGGACC
121TGGGCGGAAT GGGGTGTTCG TCTCCACCCT GCGAGTGCCA TCAGGAGGAG GACTTCAGAG
181TCACCTGCAA GGATATTCAA CGCATCCCCA GCTTACCGCC CAGTACGCAG ACTCTGAAGC
241TTATTGAGAC TCACCTGAGA ACTATTCCAA GTCATGCATT TTCTAATCTG CCCAATATTT
301CCAGAATCTA CGTATCTATA GATGTGACTC TGCAGCAGCT GGAATCACAC TCCTTCTACA
361ATTTGAGTAA AGTGACTCAC ATAGAAATTC GGAATACCAG GAACTTAACT TACATAGACC
421CTGATGCCCT CAAAGAGCTC CCCCTCCTAA AGTTCCTTGG CATTTTCAAC ACTGGACTTA
481AAATGTTCCC TGACCTGACC AAAGTTTATT CCACTGATAT ATTCTTTATA CTTGAAATTA
541CAGACAACCC TTACATGACG TCAATCCCTG TGAATGCTTT TCAGGGACTA TGCAATGAAA
601CCTTGACACT GAAGCTGTAC AACAATGGCT TTACTTCAGT CCAAGGATAT GCTTTCAATG
661GGACAAAGCT GGATGCTGTT TACCTAAACA AGAATAAATA CCTGACAGTT ATTGACAAAG
721ATGCATTTGG AGGAGTATAC AGTGGACCAA GCTTGCTGCT GCCTCTTGGA AGAAAGTCCT
781TGTCCTTTGA GACTCAGAAG GCCCCAAGCT CCAGTATGCC ATCATGCCCA ACTTTCTTGT
841ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)