Construct: ORF ccsbBroadEn_07215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002066.1_s300c1, BRDN0000456422
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COLQ (8292)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8292 | COLQ | collagen like tail subunit ... | NM_005677.4 | 99.9% | 100% | 144C>T |
| 2 | human | 8292 | COLQ | collagen like tail subunit ... | NM_080538.2 | 94.9% | 93.8% | (many diffs) |
| 3 | human | 8292 | COLQ | collagen like tail subunit ... | NM_080539.4 | 92.4% | 92.5% | 144C>T;218_219ins102 |
| 4 | mouse | 382864 | Colq | collagen-like tail subunit ... | NM_009937.2 | 88.5% | 90.6% | (many diffs) |
| 5 | mouse | 382864 | Colq | collagen-like tail subunit ... | XM_006519193.3 | 84.8% | 82.8% | (many diffs) |
| 6 | mouse | 382864 | Colq | collagen-like tail subunit ... | XM_011245104.1 | 81.1% | 83.1% | (many diffs) |
| 7 | mouse | 382864 | Colq | collagen-like tail subunit ... | XM_006519194.1 | 78.1% | 79.6% | (many diffs) |
| 8 | mouse | 382864 | Colq | collagen-like tail subunit ... | XM_011245105.1 | 78.1% | 79.6% | (many diffs) |
| 9 | mouse | 382864 | Colq | collagen-like tail subunit ... | XM_011245106.1 | 78.1% | 79.6% | (many diffs) |
| 10 | mouse | 382864 | Colq | collagen-like tail subunit ... | XM_006519195.3 | 56.5% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1434
- ORF length:
- 1365
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGTTGTCCTG AATCCAATGA CTTTGGGAAT TTATCTTCAG CTTTTCTTCC 121 TCTCTATCGT GTCTCAGCCG ACTTTCATCA ACAGCGTTCT TCCAATCTCA GCAGCCCTTC 181 CCAGCCTGGA TCAGAAGAAG CGTGGTGGCC ATAAAGCATG CTGCCTGCTG ACGCCTCCTC 241 CACCACCACT GTTCCCACCA CCATTCTTCA GAGGTGGCCG AAGTCCGCTT CTCTCCCCAG 301 ACATGAAGAA TCTCATGCTG GAACTGGAGA CCTCGCAGTC CCCGTGCATG CAAGGCTCGC 361 TAGGCTCCCC TGGGCCTCCC GGCCCCCAGG GTCCACCGGG GCTTCCTGGC AAGACAGGAC 421 CAAAGGGAGA AAAGGGGGAG CTTGGCCGAC CAGGAAGGAA GGGTAGACCT GGCCCCCCAG 481 GTGTTCCTGG CATGCCTGGG CCCATCGGTT GGCCAGGCCC TGAAGGACCC AGGGGTGAAA 541 AAGGTGACCT GGGTATGATG GGCTTGCCAG GGTCAAGAGG ACCAATGGGC TCCAAGGGCT 601 ACCCTGGATC CAGAGGGGAA AAGGGATCCA GAGGTGAAAA GGGTGACCTG GGTCCCAAAG 661 GAGAAAAGGG TTTCCCAGGA TTTCCTGGAA TGTTGGGGCA GAAAGGTGAA ATGGGTCCAA 721 AAGGTGAACC TGGGATAGCA GGACACCGAG GACCCACAGG AAGACCAGGA AAACGAGGCA 781 AGCAGGGACA GAAAGGGGAT AGTGGAGTTA TGGGCCCACC AGGCAAGCCT GGGCCTTCTG 841 GTCAACCTGG CCGTCCGGGG CCCCCAGGCC CCCCACCTGC AGGACAACTT ATAATGGGAC 901 CCAAAGGGGA AAGAGGATTT CCCGGGCCTC CAGGAAGATG TCTTTGTGGA CCCACTATGA 961 ATGTGAATAA CCCTTCCTAC GGGGAATCTG TGTATGGGCC CAGTTCCCCG CGAGTTCCTG 1021 TGATTTTTGT GGTCAACAAC CAGGAGGAGC TTGAGAGGCT GAACACCCAA AACGCCATTG 1081 CCTTCCGCAG AGACCAGAGA TCTCTGTACT TCAAGGACAG CCTTGGCTGG CTCCCCATCC 1141 AGCTGACCCC TTTCTACCCT GTGGATTACA CTGCAGACCA GCACGGCACC TGTGGGGATG 1201 GGCTCCTGCA GCCTGGGGAG GAGTGTGACG ACGGTAACAG CGATGTGGGT GACGACTGCA 1261 TCCGCTGTCA CCGTGCCTAC TGTGGAGATG GTCACCGGCA TGAGGGTGTG GAGGACTGTG 1321 ACGGCTCTGA CTTTGGCTAC CTGACATGCG AGACCTATCT CCCTGGGTCA TATGGAGACC 1381 TGCAATGCAC CCAGTACTGC TACATCGACT CCACGCCCTG CCGCTACTTC ACCTTGCCAA 1441 CTTTCTTGTA CAAAGTTGGc attataagaa agcattgctt atcaatttgt tgcaacgaac