Construct: ORF ccsbBroadEn_07599

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011097.1_s300c1, BRDN0000393918
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PEMT (10400)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10400 PEMT phosphatidylethanolamine N-... NM_148172.3 99.8% 100% 165C>T
2 human 10400 PEMT phosphatidylethanolamine N-... NM_001267551.2 90.5% 90.6% 28_29ins66;99C>T
3 human 10400 PEMT phosphatidylethanolamine N-... NM_001267552.2 89.8% 75.9% 165C>T;579_609del;696_697ins43
4 human 10400 PEMT phosphatidylethanolamine N-... XM_006721418.4 89.1% 87.2% (many diffs)
5 human 10400 PEMT phosphatidylethanolamine N-... NM_007169.3 84.1% 84.3% 0_1ins111;54C>T
6 human 10400 PEMT phosphatidylethanolamine N-... NM_148173.2 84.1% 84.3% 0_1ins111;54C>T
7 human 10400 PEMT phosphatidylethanolamine N-... XM_024450532.1 84.1% 84.3% 0_1ins111;54C>T
8 human 10400 PEMT phosphatidylethanolamine N-... XM_017024016.1 52.9% 52.9% 0_1ins333
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
774
ORF length:
708
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GAGATCTGGG AACCCGGGAG CCGAGGTAAC GAACAGCTCG GTGGCAGGGC
121CTGACTGCTG CGGAGGCCTC GGCAATATTG ATTTTAGACA GGCAGACTTC TGCGTTATGA
181CCCGGCTGCT GGGCTACGTG GACCCCCTGG ATCCCAGCTT TGTGGCTGCT GTCATCACCA
241TCACCTTCAA TCCGCTCTAC TGGAATGTGG TTGCACGATG GGAACACAAG ACCCGCAAGC
301TGAGCAGGGC CTTCGGATCC CCCTACCTGG CCTGCTACTC TCTAAGCGTC ACCATCCTGC
361TCCTGAACTT CCTGCGCTCG CACTGCTTCA CGCAGGCCAT GCTGAGCCAG CCCAGGATGG
421AGAGCCTGGA CACCCCCGCG GCCTACAGCC TGGGCCTCGC GCTCCTGGGA CTGGGCGTCG
481TGCTCGTGCT CTCCAGCTTC TTTGCACTGG GGTTCGCTGG AACTTTCCTA GGTGATTACT
541TCGGGATCCT CAAGGAGGCG AGAGTGACCG TGTTCCCCTT CAACATCCTG GACAACCCCA
601TGTACTGGGG AAGCACAGCC AACTACCTGG GCTGGGCCAT CATGCACGCC AGCCCCACGG
661GCCTGCTCCT GACGGTGCTG GTGGCCCTCA CCTACATAGT GGCTCTCCTA TACGAAGAGC
721CCTTCACCGC TGAGATCTAC CGGCAGAAAG CCTCCGGGTC CCACAAGAGG AGCTGCCCAA
781CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)