Transcript: Human NM_148172.3

Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
PEMT (10400)
Length:
1021
CDS:
83..793

Additional Resources:

NCBI RefSeq record:
NM_148172.3
NBCI Gene record:
PEMT (10400)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_148172.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035477 CCGCTCTACTGGAATGTGGTT pLKO.1 269 CDS 100% 2.640 3.696 N PEMT n/a
2 TRCN0000288827 CCGCTCTACTGGAATGTGGTT pLKO_005 269 CDS 100% 2.640 3.696 N PEMT n/a
3 TRCN0000035475 CTACTCTCTAAGCGTCACCAT pLKO.1 352 CDS 100% 2.640 3.696 N PEMT n/a
4 TRCN0000288758 CTACTCTCTAAGCGTCACCAT pLKO_005 352 CDS 100% 2.640 3.696 N PEMT n/a
5 TRCN0000035476 GCTGAGATCTACCGGCAGAAA pLKO.1 746 CDS 100% 4.950 3.465 N PEMT n/a
6 TRCN0000288825 GCTGAGATCTACCGGCAGAAA pLKO_005 746 CDS 100% 4.950 3.465 N PEMT n/a
7 TRCN0000035474 CCTAGGTGATTACTTCGGGAT pLKO.1 544 CDS 100% 2.160 1.512 N PEMT n/a
8 TRCN0000288826 CCTAGGTGATTACTTCGGGAT pLKO_005 544 CDS 100% 2.160 1.512 N PEMT n/a
9 TRCN0000035478 GCCCTCACCTACATAGTGGCT pLKO.1 701 CDS 100% 0.220 0.154 N PEMT n/a
10 TRCN0000288756 GCCCTCACCTACATAGTGGCT pLKO_005 701 CDS 100% 0.220 0.154 N PEMT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_148172.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07599 pDONR223 100% 99.8% 100% None 165C>T n/a
2 ccsbBroad304_07599 pLX_304 0% 99.8% 100% V5 165C>T n/a
3 TRCN0000466328 AACATCCTAACTACGGTTTCCGAG pLX_317 56.5% 99.8% 100% V5 165C>T n/a
4 ccsbBroadEn_07598 pDONR223 100% 99.7% 99.1% None 283G>A;634G>A n/a
5 ccsbBroad304_07598 pLX_304 0% 99.7% 99.1% V5 283G>A;634G>A n/a
6 TRCN0000480159 AGATCCTCCGTTAGCACCAACTTT pLX_317 57.8% 99.7% 99.1% V5 283G>A;634G>A n/a
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