Construct: ORF ccsbBroadEn_07790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006943.1_s300c1, BRDN0000386000
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNF8 (11267)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11267 | SNF8 | SNF8 subunit of ESCRT-II | NM_007241.4 | 99.8% | 99.6% | 11G>T |
| 2 | human | 11267 | SNF8 | SNF8 subunit of ESCRT-II | NM_001317192.2 | 99.4% | 99.2% | 11G>T;562_563insAGA |
| 3 | human | 11267 | SNF8 | SNF8 subunit of ESCRT-II | NM_001317193.2 | 93.2% | 93% | 11G>T;52_53ins51 |
| 4 | human | 11267 | SNF8 | SNF8 subunit of ESCRT-II | NM_001317194.2 | 58.7% | 55.8% | (many diffs) |
| 5 | human | 11267 | SNF8 | SNF8 subunit of ESCRT-II | XM_017024075.2 | 58.7% | 55.8% | (many diffs) |
| 6 | human | 11267 | SNF8 | SNF8 subunit of ESCRT-II | NR_133679.2 | 37.1% | (many diffs) | |
| 7 | mouse | 27681 | Snf8 | SNF8, ESCRT-II complex subu... | NM_033568.2 | 90.6% | 98% | (many diffs) |
| 8 | mouse | 27681 | Snf8 | SNF8, ESCRT-II complex subu... | XM_006533468.1 | 82% | 88.7% | (many diffs) |
| 9 | mouse | 27681 | Snf8 | SNF8, ESCRT-II complex subu... | XM_006533467.1 | 79.7% | 75.6% | (many diffs) |
| 10 | mouse | 27681 | Snf8 | SNF8, ESCRT-II complex subu... | XM_017314573.1 | 51.6% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCA CCGCCTCGGG GTGGGAGCTG GCGCCATCGC CAAGAAGAAA CTTGCAGAGG 121 CCAAGTATAA GGAGCGAGGG ACGGTCTTGG CTGAGGACCA GCTAGCCCAG ATGTCAAAGC 181 AGTTGGACAT GTTCAAGACC AACCTGGAGG AATTTGCCAG CAAACACAAG CAGGAGATCC 241 GGAAGAATCC TGAGTTCCGT GTGCAGTTCC AGGACATGTG TGCAACCATT GGCGTGGATC 301 CGCTGGCCTC TGGAAAAGGA TTTTGGTCTG AGATGCTGGG CGTGGGGGAC TTCTATTACG 361 AACTAGGTGT CCAAATTATC GAAGTGTGCC TGGCGCTGAA GCATCGGAAT GGAGGTCTGA 421 TAACTTTGGA GGAACTACAT CAACAGGTGT TGAAGGGAAG GGGCAAGTTC GCCCAGGATG 481 TCAGTCAAGA TGACCTGATC AGAGCCATCA AGAAACTAAA GGCACTTGGC ACTGGCTTCG 541 GCATCATCCC TGTGGGCGGC ACTTACCTCA TTCAGTCTGT TCCAGCTGAG CTCAATATGG 601 ATCACACCGT GGTGCTGCAG CTGGCAGAGA AGAATGGCTA CGTGACTGTC AGTGAGATCA 661 AAGCCAGTCT TAAATGGGAG ACCGAGCGAG CGCGGCAAGT GCTGGAACAC CTGCTGAAGG 721 AAGGGTTGGC GTGGCTGGAC TTACAGGCCC CAGGGGAGGC CCACTACTGG CTGCCAGCTC 781 TCTTCACTGA CCTCTACTCC CAGGAGATTA CAGCTGAGGA GGCCAGAGAA GCCCTCCCCT 841 GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 901 acgaac