Construct: ORF ccsbBroadEn_07813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001398.1_s300c1, BRDN0000387241
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CARD8 (22900)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001184902.2 | 98.5% | 98.4% | 246T>C;758T>C;1162_1176del |
2 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001184903.1 | 98.5% | 98.4% | 246T>C;758T>C;1162_1176del |
3 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723106.4 | 98.5% | 98.4% | 246T>C;758T>C;1162_1176del |
4 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526650.2 | 98.5% | 98.4% | 246T>C;758T>C;1162_1176del |
5 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723107.3 | 90.1% | 89.1% | (many diffs) |
6 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351788.2 | 85.7% | 85.7% | (many diffs) |
7 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351789.2 | 85.7% | 85.7% | (many diffs) |
8 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026492.2 | 85.7% | 85.7% | (many diffs) |
9 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026485.1 | 83.2% | 83.1% | 246T>C;758T>C;1162_1392del |
10 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351790.2 | 77.8% | 68% | (many diffs) |
11 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001184900.3 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
12 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351782.2 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
13 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723091.4 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
14 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723092.4 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
15 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723093.4 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
16 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526641.3 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
17 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526643.3 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
18 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526644.3 | 71.9% | 71.8% | 246T>C;758T>C;1162_1611del |
19 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351791.2 | 71.8% | 66.8% | (many diffs) |
20 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351792.2 | 71.8% | 66.8% | (many diffs) |
21 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723094.4 | 71.7% | 71.6% | (many diffs) |
22 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026482.2 | 71.7% | 71.6% | (many diffs) |
23 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723109.3 | 71.5% | 66.5% | (many diffs) |
24 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026498.1 | 71.5% | 66.5% | (many diffs) |
25 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026499.1 | 69.3% | 68.8% | (many diffs) |
26 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001184901.1 | 62.6% | 62.5% | (many diffs) |
27 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351783.2 | 62.6% | 62.5% | (many diffs) |
28 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_014959.5 | 62.6% | 62.5% | (many diffs) |
29 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723096.4 | 62.6% | 62.5% | (many diffs) |
30 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723097.4 | 62.6% | 62.5% | (many diffs) |
31 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026483.2 | 62.6% | 62.5% | (many diffs) |
32 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026484.2 | 62.4% | 62.3% | (many diffs) |
33 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351787.1 | 60.7% | 56.4% | (many diffs) |
34 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526646.3 | 58% | 50% | (many diffs) |
35 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526647.3 | 58% | 50% | (many diffs) |
36 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_011526648.3 | 57.8% | 49.8% | (many diffs) |
37 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723098.3 | 56.8% | 49.8% | (many diffs) |
38 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_024451421.1 | 56.8% | 49.8% | (many diffs) |
39 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_024451422.1 | 56.6% | 49.6% | (many diffs) |
40 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351784.2 | 52.4% | 48.7% | (many diffs) |
41 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723102.4 | 52.4% | 48.7% | (many diffs) |
42 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_024451423.1 | 52.4% | 48.7% | (many diffs) |
43 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001365950.1 | 52.2% | 48.6% | (many diffs) |
44 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723104.3 | 52.2% | 48.6% | (many diffs) |
45 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001351786.2 | 50.8% | 50.2% | (many diffs) |
46 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026487.2 | 50.8% | 50.2% | (many diffs) |
47 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_017026488.2 | 50.8% | 50.2% | (many diffs) |
48 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_024451424.1 | 50.8% | 50.4% | (many diffs) |
49 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_024451425.1 | 50.8% | 50.4% | (many diffs) |
50 | human | 22900 | CARD8 | caspase recruitment domain ... | XR_001753636.1 | 41.1% | (many diffs) | |
51 | human | 22900 | CARD8 | caspase recruitment domain ... | XR_001753637.2 | 37.9% | (many diffs) | |
52 | human | 22900 | CARD8 | caspase recruitment domain ... | NR_147707.1 | 34% | (many diffs) | |
53 | human | 22900 | CARD8 | caspase recruitment domain ... | XR_430193.3 | 21.9% | (many diffs) | |
54 | human | 22900 | CARD8 | caspase recruitment domain ... | NR_033679.1 | 21.1% | (many diffs) | |
55 | human | 22900 | CARD8 | caspase recruitment domain ... | NR_033680.1 | 18.3% | (many diffs) | |
56 | human | 22900 | CARD8 | caspase recruitment domain ... | NM_001184904.1 | 18% | 18% | 210_211ins951 |
57 | human | 22900 | CARD8 | caspase recruitment domain ... | XM_006723110.3 | 18% | 18% | 210_211ins951 |
58 | human | 22900 | CARD8 | caspase recruitment domain ... | NR_033678.1 | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA AAAAAAGGAG TGTCCAGAAA AGAGTAGCAG CAGTGAGGAA GAGCTGCCGA 121 GACGGGACAG TGGATCCAGT AGGAACATAG ATGCATCCAA ACTCATTAGA CTACAAGGAT 181 CACGGAAACT GTTGGTTGAC AATAGCATAC GGGAACTGCA ATACACAAAA ACTGGAATTT 241 TTTTTCAGGC TGAGGCCTGT GTGACAAATG ATACGGTATA CAGGGAGCTA CCCTGTGTTT 301 CTGAGACCCT CTGTGACATC TCACATTTTT TCCAAGAAGA TGATGAGACA GAGGCAGAGC 361 CATTATTGTT CCGTGCTGTT CCTGAGTGTC AACTATCTGG GGGGGACATT CCCAGTGTAT 421 CAGAAGAGCA GGAATCTTCA GAGGGACAAG ATTCAGGAGA CATTTGCTCA GAAGAGAATC 481 AAATAGTTTC CTCTTATGCT TCTAAAGTCT GTTTTGAGAT CGAAGAAGAT TATAAAAATC 541 GTCAGTTTCT GGGGCCTGAA GGAAATGTGG ATGTTGAGTT GATTGATAAG AGCACAAACA 601 GATACAGCGT TTGGTTCCCC ACTGCTGGCT GGTATCTGTG GTCAGCCACA GGCCTCGGCT 661 TCCTGGTAAG GGATGAGGTC ACAGTGACGA TTGCGTTTGG TTCCTGGAGT CAGCACCTGG 721 CCCTGGACCT GCAGCACCAT GAACAGTGGC TGGTGGGCGG CCCCTTGTTT GATGTCACTG 781 CAGAGCCAGA GGAGGCTGTC GCCGAAATCC ACCTCCCCCA CTCCATCTCC CTCCAAGCAG 841 GTGAGGTGGA CGTCTCCTGG TTTCTCGTTG CCCATTTTAA GAATGAAGGG ATGGTCCTGG 901 AGCATCCAGC CCGGGTGGAG CCTTTCTATG CTGTCCTGGA AAGCCCCAGC TTCTCTCTGA 961 TGGGCATCCT GCTGCGGATC GCCAGTGGGA CTCGCCTCTC CATCCCCATC ACTTCCAACA 1021 CATTGATCTA TTATCACCCC CACCCCGAAG ATATTAAGTT CCACTTGTAC CTTGTCCCCA 1081 GCGACGCCTT GCTAACAAAG GCGATAGATG ATGAGGAAGA TCGCTTCCAT GGTGTGCGCC 1141 TGCAGACTTC GCCCCCAATG GAACCCCTGA ACTTTGGTTC CAGTTATATT GTGTCTAATT 1201 CTGCTAACCT GAAAGTAATG CCCAAGTGGA TCTCCAGCTT GTACCCAACT TTCTTGTACA 1261 AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac