Construct: ORF ccsbBroadEn_07869

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005189.1_s300c1, BRDN0000385992
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SUN1 (23353)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001171946.2 99.8% 99.6% 610G>C
2 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001171945.2 92.3% 92% 1_63del;673G>C
3 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367637.1 44.2% 39.5% (many diffs)
4 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367687.1 32.4% 29.3% (many diffs)
5 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367665.1 32.4% 28.3% (many diffs)
6 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367653.1 32.3% 28.3% (many diffs)
7 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001171944.2 32.2% 22.2% (many diffs)
8 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367702.1 32% 28.7% (many diffs)
9 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367672.1 31.5% 27.6% (many diffs)
10 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367642.1 31.3% 29.2% (many diffs)
11 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367694.1 31.1% 27.3% (many diffs)
12 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001130965.3 31% 27.2% (many diffs)
13 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367633.1 31% 27.2% (many diffs)
14 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367634.1 31% 27.2% (many diffs)
15 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367704.1 31% 27.9% (many diffs)
16 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367669.1 30.7% 26.9% (many diffs)
17 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367685.1 29.9% 27.8% (many diffs)
18 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367693.1 29.6% 26.1% (many diffs)
19 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367675.1 29.3% 26.5% (many diffs)
20 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367696.1 29.2% 27.1% (many diffs)
21 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367688.1 29% 26.3% (many diffs)
22 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367683.1 29% 26.3% (many diffs)
23 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367640.1 28.9% 26.2% (many diffs)
24 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367676.1 28.9% 26.2% (many diffs)
25 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367682.1 28.8% 26.1% (many diffs)
26 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367638.1 28.8% 26.8% (many diffs)
27 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367664.1 28.8% 26.8% (many diffs)
28 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367674.1 28.7% 26% (many diffs)
29 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367643.1 28.6% 25.2% (many diffs)
30 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367700.1 28.6% 25.9% (many diffs)
31 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367697.1 28.6% 25.2% (many diffs)
32 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367698.1 27.8% 25.2% (many diffs)
33 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367641.1 27.8% 25.2% (many diffs)
34 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367690.1 27.7% 25.1% (many diffs)
35 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367699.1 27.7% 25.1% (many diffs)
36 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367703.1 27.6% 25% (many diffs)
37 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367677.1 27.6% 25% (many diffs)
38 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367692.1 27.5% 25% (many diffs)
39 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367678.1 26.7% 24.2% (many diffs)
40 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367705.1 26.7% 24.2% (many diffs)
41 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367662.1 25.7% 21.9% (many diffs)
42 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_025154.6 25.2% 23.3% (many diffs)
43 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367660.1 25% 21.4% (many diffs)
44 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367686.1 24.9% 22.8% (many diffs)
45 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367671.1 24.7% 21.1% (many diffs)
46 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367648.1 24.7% 21.7% (many diffs)
47 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367670.1 24.7% 21.1% (many diffs)
48 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367651.1 24.3% 21.2% (many diffs)
49 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367667.1 23.7% 21.6% (many diffs)
50 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367647.1 23.6% 20.2% (many diffs)
51 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367701.1 23.2% 20.6% (many diffs)
52 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367644.1 23% 20.3% (many diffs)
53 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367706.1 23% 20.3% (many diffs)
54 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367680.1 22.9% 20.3% (many diffs)
55 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367681.1 22.9% 20.2% (many diffs)
56 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367679.1 22.8% 20.9% (many diffs)
57 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367689.1 22.8% 20.8% (many diffs)
58 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367668.1 22.7% 19.4% (many diffs)
59 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367649.1 22.7% 19.4% (many diffs)
60 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367646.1 22.2% 19.6% (many diffs)
61 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367673.1 22.1% 19.6% (many diffs)
62 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367645.1 22% 20.1% (many diffs)
63 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367636.1 22% 19.5% (many diffs)
64 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367684.1 21.9% 19.4% (many diffs)
65 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367655.1 21.5% 19% (many diffs)
66 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367691.1 21.3% 18.8% (many diffs)
67 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367666.1 21.2% 18.8% (many diffs)
68 human 23353 SUN1 Sad1 and UNC84 domain conta... NM_001367695.1 21.2% 19.3% (many diffs)
69 human 23353 SUN1 Sad1 and UNC84 domain conta... NR_160281.1 18.2% (many diffs)
70 human 23353 SUN1 Sad1 and UNC84 domain conta... NR_160283.1 17.9% (many diffs)
71 human 23353 SUN1 Sad1 and UNC84 domain conta... NR_160282.1 16.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
837
ORF length:
771
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA TTTTTCTCGG CTTCACATGT ACAGTCCTCC CCAGTGTGTG CCGGAGAACA
121CGGGCTACAC GTATGCGCTC AGTTCCAGCT ATTCTTCAGA TGCTCTGGAT TTTGAGACGG
181AGCACAAATT GGACCCTGTA TTTGATTCTC CACGGATGTC CCGCCGTAGT TTGCGCCTGG
241CCACGACAGC ATGCACCCTG GGGGATGGTG AGGCTGTGGG TGCCGACAGC GGCACCAGCA
301GCGCTGTCTC CCTGAAGAAC CGAGCGGCCA GAACAACAAA ACAGCGCAGA AGCACAAACA
361AATCAGCTTT TAGTATCAAC CACGTGTCAA GGCAGGTCAC GTCCTCTGGC GTCAGCCACG
421GCGGCACTGT CAGCCTGCAG GATGCTGTGA CTCGACGGCC TCCTGTATTG GACGAGTCTT
481GGATTCGTGA ACAGACCACA GTGGACCACT TCTGGGGTCT TGATGATGAT GGTGATCTTA
541AAGGTGGAAA TAAAGCTGCC ATTCAGGGAA ACGGGGATGT GGGAGCCGCC GCCGCCACCG
601CGCACAACGG CTTCTCCTGC AGCAACTGCA GCATGCTGTC CGAGCGCAAG GACGTGCTCA
661CGGCGCACCC CGCGCCCCCC GGGCCCGTGT CGAGAGTTTA TTCTAGGGAC AGGAATCAAA
721AATGTAAGTC TCAGTCCTTT AAAACTCAGA AAAAGGTGTG TTTTCCAAAT TTAATATTTC
781CTTTCTGTAA GTCTCAGTGT CTGCACTATT TGTCTTGGAG ACTTAAAATT ATCCCTTGCC
841CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
901aac

Download FASTA (ORF) (Full)