Construct: ORF ccsbBroadEn_07982

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013542.1_s300c1, BRDN0000393254
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APPL1 (26060)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26060 APPL1 adaptor protein, phosphotyr... NM_012096.3 99.9% 100% 69A>G
2 human 26060 APPL1 adaptor protein, phosphotyr... XM_011533583.3 97.4% 97.4% 0_1ins52;2delT;18A>G
3 mouse 72993 Appl1 adaptor protein, phosphotyr... NM_145221.2 92.1% 98% (many diffs)
4 mouse 72993 Appl1 adaptor protein, phosphotyr... XM_006519608.1 89% 94.6% (many diffs)
5 mouse 72993 Appl1 adaptor protein, phosphotyr... XM_017316214.1 77.7% 82.5% (many diffs)
6 mouse 72993 Appl1 adaptor protein, phosphotyr... XM_006519609.3 69.3% 73.2% (many diffs)
7 mouse 72993 Appl1 adaptor protein, phosphotyr... XR_001781158.1 59.6% (many diffs)
8 mouse 72993 Appl1 adaptor protein, phosphotyr... XR_001781160.1 45.7% (many diffs)
9 mouse 72993 Appl1 adaptor protein, phosphotyr... XR_001781159.1 24.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2193
ORF length:
2127
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC GGGGATCGAC AAGCTGCCCA TCGAGGAGAC GCTGGAGGAC AGCCCGCAGA
121CAAGGTCTTT ACTGGGTGTA TTTGAAGAAG ATGCCACAGC TATTTCCAAC TATATGAACC
181AGTTGTATCA AGCTATGCAT CGGATTTATG ATGCACAGAA TGAATTAAGT GCAGCAACAC
241ACCTGACCTC AAAACTTTTA AAAGAATATG AAAAACAGCG TTTTCCATTG GGAGGTGATG
301ATGAAGTTAT GAGCTCTACA TTGCAACAGT TTTCAAAAGT TATAGATGAG CTTAGCTCTT
361GTCATGCAGT GCTTTCAACT CAACTTGCTG ATGCCATGAT GTTCCCCATT ACCCAGTTTA
421AAGAAAGAGA TCTGAAAGAA ATACTAACAT TAAAGGAAGT ATTTCAGATT GCAAGTAATG
481ATCATGATGC TGCGATTAAT AGATATAGCC GTTTATCAAA AAAAAGAGAA AATGACAAGG
541TGAAGTATGA AGTAACAGAA GATGTGTACA CATCCAGAAA GAAACAACAC CAGACCATGA
601TGCATTATTT TTGTGCATTA AATACTCTTC AGTACAAGAA GAAAATAGCA TTGTTAGAAC
661CTCTACTTGG GTACATGCAA GCTCAGATAA GTTTCTTTAA GATGGGTTCT GAAAATCTTA
721ATGAACAACT GGAAGAATTT TTAGCTAATA TTGGAACAAG CGTTCAGAAT GTTCGCAGGG
781AAATGGACAG TGATATAGAG ACCATGCAAC AGACAATAGA GGATTTGGAA GTAGCCAGTG
841ATCCCTTATA TGTGCCTGAC CCAGACCCCA CCAAATTTCC TGTTAATCGA AATTTAACCC
901GAAAGGCTGG ATACCTTAAT GCTAGGAATA AAACAGGCTT GGTGTCATCT ACCTGGGACA
961GACAGTTTTA CTTCACGCAG GGTGGAAATT TAATGAGTCA GGCCCGTGGG GATGTAGCAG
1021GAGGCCTGGC CATGGACATA GACAACTGTT CAGTGATGGC TGTGGACTGT GAAGACAGAC
1081GATATTGTTT TCAGATCACC TCTTTCGATG GAAAAAAATC TTCAATTTTG CAAGCAGAGA
1141GTAAAAAAGA TCATGAAGAG TGGATCTGTA CAATAAACAA CATATCTAAA CAAATATACT
1201TAAGTGAAAA TCCAGAGGAA ACTGCTGCAC GAGTAAATCA ATCAGCTCTG GAAGCTGTCA
1261CTCCTTCCCC ATCTTTCCAG CAGAGGCACG AGAGCCTGCG GCCAGCAGCA GGACAATCTC
1321GGCCACCGAC AGCTCGAACC AGCAGTTCAG GATCCTTAGG ATCTGAGTCT ACAAATTTGG
1381CTGCCCTCTC TCTAGATTCT CTTGTTGCCC CAGACACCCC AATACAGTTT GACATAATTT
1441CTCCTGTGTG TGAAGATCAG CCTGGCCAGG CAAAAGCCTT TGGCCAGGGA GGCAGGCGTA
1501CAAATCCATT TGGAGAATCT GGAGGAAGTA CAAAATCTGA AACTGAAGAT TCTATTCTTC
1561ATCAGTTATT TATTGTCCGA TTCCTTGGTT CAATGGAGGT GAAATCAGAT GACCATCCAG
1621ATGTTGTTTA TGAAACTATG CGCCAAATCT TAGCTGCCCG GGCCATCCAT AACATCTTTC
1681GTATGACAGA ATCGCATTTA TTAGTCACTT GTGACTGTTT AAAGTTAATT GATCCACAGA
1741CACAAGTTAC AAGGCTCACG TTTCCATTAC CTTGTGTAGT TTTGTATGCT ACACACCAGG
1801AAAATAAGCG CCTTTTTGGA TTTGTTCTTC GGACATCAAG CGGGAGAAGT GAAAGTAATC
1861TGTCATCAGT CTGCTATATA TTTGAGTCAA ACAATGAGGG GGAAAAGATA TGTGATTCTG
1921TTGGACTGGC AAAACAGATA GCTTTGCATG CTGAACTGGA TCGTAGGGCA TCAGAAAAAC
1981AAAAAGAAAT AGAGAGAGTA AAAGAGAAGC AACAGAAAGA ACTCAATAAA CAAAAACAGA
2041TTGAAAAGGA CTTGGAAGAA CAAAGTCGGT TGATAGCTGC TTCCAGTAGA CCAAACCAAG
2101CCAGTAGTGA GGGGCAGTTT GTTGTCCTTA GCAGTAGCCA GTCAGAAGAG AGTGATTTGG
2161GAGAAGGAGG AAAGAAGAGA GAATCAGAAG CATACCCAAC TTTCTTGTAC AAAGTtggca
2221ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)