Construct: ORF ccsbBroadEn_08170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012239.1_s300c1, BRDN0000395131
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3R4 (30849)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30849 | PIK3R4 | phosphoinositide-3-kinase r... | NM_014602.3 | 99.9% | 99.9% | 692C>G |
| 2 | mouse | 75669 | Pik3r4 | phosphoinositide-3-kinase r... | NM_001081309.1 | 85.6% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4143
- ORF length:
- 4074
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGGAAATCAG CTTGCTGGCA TTGCTCCCTC CCAGATCCTT TCTGTAGAGA 121 GTTATTTTTC AGATATTCAT GACTTTGAAT ATGATAAAAG CCTGGGGAGT ACTCGGTTTT 181 TTAAAGTTGC TCGAGCCAAG CACCGAGAAG GCCTGGTCGT TGTGAAGGTT TTTGCAATTC 241 AGGATCCCAC ATTGCCTTTA ACCAGCTATA AACAAGAGCT GGAGGAACTG AAAATCAGGC 301 TTAATTCTGC ACAGAATTGT CTACCTTTCC AGAAAGCATC AGAAAAAGCA TCTGAGAAAG 361 CAGCTATGCT CTTTAGGCAG TATGTGCGAG ACAATCTCTA TGATCGCATC AGTACCCGTC 421 CATTCTTGAA TAACATTGAG AAGCGCTGGA TTGCTTTCCA GATCCTGACA GCTGTGGACC 481 AAGCACACAA ATCTGGAGTT CGTCATGGGG ACATCAAGAC TGAGAATGTG ATGGTCACCA 541 GTTGGAATTG GGTTCTTCTA ACTGATTTTG CCAGTTTTAA GCCCACTTAT CTTCCAGAAG 601 ACAACCCGGC AGATTTCAAT TATTTCTTTG ACACATCACG GAGGAGAACT TGCTATATTG 661 CTCCTGAACG TTTTGTTGAT GGTGGGATGT TTGCCACTGA GTTAGAATAT ATGAGAGATC 721 CTTCAACTCC GCTTGTAGAC TTAAATAGCA ATCAGAGAAG AAGAGGAGAG TTGAAGAGAG 781 CAATGGACAT CTTTTCAGCA GGTTGTGTGA TAGCTGAGCT TTTTACAGAA GGTGTACCAT 841 TATTTGATCT CTCTCAACTT TTGGCTTATA GAAATGGACA TTTTTTCCCT GAACAAGTGC 901 TAAATAAAAT TGAAGATCAC AGTATCAGAG AATTGGTAAC TCAGATGATT CACCGTGAGC 961 CAGATAAACG TTTAGAGGCA GAAGATTACT TAAAACAGCA GCGTGGCAAT GCCTTTCCTG 1021 AAATATTTTA CACTTTTCTT CAGCCCTACA TGGCCCAGTT TGCCAAGGAA ACGTTTCTTT 1081 CTGCAGATGA GCGTATTCTG GTTATACGGA AGGATTTGGG CAACATTATT CACAATCTCT 1141 GTGGACATGA TCTGCCAGAA AAAGCCGAAG GAGAGCCTAA GGAAAATGGG CTGGTTATCT 1201 TGGTATCTGT TATAACATCC TGCCTACAGA CCCTTAAATA CTGTGATTCC AAACTAGCTG 1261 CTTTGGAACT GATTCTTCAT TTGGCTCCAA GATTAAGTGT TGAAATCCTT TTGGATCGTA 1321 TTACTCCATA TCTTTTGCAT TTCAGCAATG ACTCTGTTCC TAGGGTGAGG GCTGAAGCCT 1381 TGAGGACGTT GACCAAAGTT CTTGCTCTCG TCAAAGAGGT TCCTCGTAAT GATATCAATA 1441 TTTATCCGGA ATACATTCTG CCAGGCATAG CCCACTTAGC CCAAGATGAT GCTACTATCG 1501 TTAGACTAGC CTATGCTGAA AACATAGCTC TGCTGGCAGA AACAGCTCTG AGATTCCTGG 1561 AATTAGTACA GTTAAAAAAT CTTAATATGG AAAATGACCC CAATAATGAA GAAATAGATG 1621 AGGTTACACA TCCAAATGGA AATTATGACA CAGAGCTCCA AGCCTTACAT GAAATGGTCC 1681 AGCAGAAAGT TGTTACTTTG CTAAGTGACC CTGAAAATAT TGTAAAACAA ACCTTGATGG 1741 AAAATGGAAT AACACGGCTG TGTGTATTCT TTGGACGTCA GAAAGCCAAC GATGTTTTGT 1801 TGTCCCACAT GATTACTTTC CTAAATGATA AGAATGATTG GCATCTACGT GGAGCATTTT 1861 TTGATAGTAT AGTTGGTGTT GCTGCCTATG TTGGCTGGCA AAGCTCCTCA ATTCTCAAGC 1921 CTCTGCTGCA ACAAGGTCTT AGTGATGCTG AGGAATTTGT CATTGTGAAA GCTCTTTATG 1981 CCCTTACTTG TATGTGCCAG TTAGGACTGC TACAAAAACC CCATGTTTAC GAATTTGCCA 2041 GTGATATTGC CCCCTTCCTG TGTCATCCCA ATTTATGGAT ACGTTATGGT GCCGTGGGAT 2101 TTATCACAGT GGTAGCTCGT CAAATAAGTA CAGCTGATGT CTACTGTAAA CTGATGCCTT 2161 ATCTTGACCC ATATATTACC CAACCAATAA TACAGATTGA AAGAAAACTT GTTCTGCTCA 2221 GTGTTTTAAA GGAACCAGTA AGTCGTTCTA TATTTGATTA TGCTTTGAGG TCTAAAGATA 2281 TTACTAGCTT GTTCAGACAT CTTCACATGC GTCAGAAGAA ACGAAATGGT TCTCTTCCCG 2341 ACTGCCCTCC GCCAGAGGAT CCTGCCATAG CACAGCTTCT GAAGAAGTTG CTCTCACAGG 2401 GAATGACAGA GGAAGAGGAA GACAAACTTC TGGCACTGAA AGACTTCATG ATGAAATCTA 2461 ATAAAGCAAA GGCCAATATA GTGGACCAGA GCCATCTTCA TGATAGTAGT CAGAAAGGTG 2521 TAATTGACTT GGCAGCTTTA GGCATAACTG GGAGACAAGT TGATCTTGTT AAAACCAAAC 2581 AAGAACCAGA TGACAAACGG GCCAGAAAAC ATGTAAAACA AGACTCAAAT GTAAATGAAG 2641 AATGGAAAAG CATGTTTGGG TCACTGGACC CACCAAACAT GCCACAGGCC CTACCTAAAG 2701 GGAGTGATCA GGAGGTGATT CAGACTGGGA AACCTCCTCG TTCCGAGTCC TCTGCTGGCA 2761 TTTGTGTCCC TTTGTCAACT TCTTCACAGG TTCCAGAAGT GACAACTGTC CAAAATAAAA 2821 AACCAGTAAT ACCGGTTTTA AGTAGTACAA TCTTACCATC CACCTATCAG ATTCGAATTA 2881 CAACTTGTAA AACTGAACTT CAGCAACTCA TCCAGCAAAA GCGGGAGCAG TGCAATGCTG 2941 AGAGAATAGC TAAGCAGATG ATGGAAAATG CTGAATGGGA GAGTAAACCA CCACCACCTG 3001 GATGGCGTCC TAAAGGGCTG TTAGTTGCCC ATCTTCATGA GCATAAATCT GCTGTGAATC 3061 GAATTAGAGT CTCTGATGAA CACTCACTTT TTGCAACATG TTCAAATGAT GGCACAGTGA 3121 AAATCTGGAA CAGTCAAAAG ATGGAGGGGA AGACCACCAC TACCAGATCT ATTCTTACAT 3181 ACAGCCGAAT TGGAGGACGA GTCAAGACGC TCACATTCTG CCAAGGCTCC CACTATTTAG 3241 CCATAGCATC TGATAATGGT GCTGTCCAGC TTCTTGGAAT TGAGGCTTCT AAGCTGCCCA 3301 AGTCTCCTAA AATCCATCCT CTACAAAGCA GAATTCTAGA TCAGAAGGAG GACGGTTGTG 3361 TTGTGGATAT GCATCACTTC AACTCTGGAG CACAGTCTGT TCTTGCCTAT GCCACTGTGA 3421 ATGGCTCTCT GGTTGGCTGG GACCTTAGGT CTTCAAGCAA TGCGTGGACT TTAAAGCATG 3481 ATTTAAAGTC GGGCCTCATC ACTTCCTTTG CTGTGGACAT CCACCAATGC TGGCTCTGCA 3541 TTGGTACAAG CAGTGGTACC ATGGCTTGTT GGGACATGAG GTTCCAGTTG CCAATTTCAA 3601 GTCACTGTCA TCCTTCCAGG GCTCGAATCA GACGCCTCTC AATGCACCCT CTGTATCAGT 3661 CCTGGGTGAT TGCAGCTGTT CAGGGCAACA ACGAAGTGTC CATGTGGGAC ATGGAGACTG 3721 GTGACAGAAG ATTTACTCTC TGGGCCAGCA GTGCACCACC ACTTTCTGAA TTACAGCCTT 3781 CTCCTCATAG CGTCCATGGT ATCTACTGTA GTCCTGCAGA TGGAAATCCT ATCCTACTAA 3841 CAGCTGGCTC AGATATGAAA ATAAGGTTTT GGGACTTGGC TTACCCAGAA AGGTCCTATG 3901 TTGTTGCAGG AAGTACTAGT TCCCCATCTG TGTCCTACTA CAGGAAAATA ATTGAAGGCA 3961 CTGAAGTTGT CCAGGAAATT CAGAATAAGC AGAAAGTAGG ACCAAGTGAT GACACCCCTC 4021 GAAGGGGCCC AGAGTCCCTG CCCGTGGGAC ATCATGACAT CATCACTGAT GTCGCCACAT 4081 TCCAGACCAC ACAGGGCTTC ATCGTAACTG CTTCTAGAGA TGGGATTGTG AAGGTGTGGA 4141 AATTGCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt 4201 gcaacgaac