Construct: ORF ccsbBroadEn_08187

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000886.1_s300c1, BRDN0000398393
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TAS2R14 (50840)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50840 TAS2R14 taste 2 receptor member 14 NM_023922.1 99.8% 100% 375G>A
2 human 106707243 PRH1-TAS2R14 PRH1-TAS2R14 readthrough NM_001316893.2 45.1% 38.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1020
ORF length:
951
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGGTGGTGTC ATAAAGAGCA TATTTACATT CGTTTTAATT GTGGAATTTA
121TAATTGGAAA TTTAGGAAAT AGTTTCATAG CACTGGTGAA CTGTATTGAC TGGGTCAAGG
181GAAGAAAGAT CTCTTCGGTT GATCGGATCC TCACTGCTTT GGCAATCTCT CGAATTAGCC
241TGGTTTGGTT AATATTCGGA AGCTGGTGTG TGTCTGTGTT TTTCCCAGCT TTATTTGCCA
301CTGAAAAAAT GTTCAGAATG CTTACTAATA TCTGGACAGT GATCAATCAT TTTAGTGTCT
361GGTTAGCTAC AGGCCTCGGT ACTTTTTATT TTCTCAAGAT AGCCAATTTT TCTAACTCTA
421TTTTTCTCTA CCTAAAGTGG AGAGTTAAAA AGGTGGTTTT GGTGCTGCTT CTTGTGACTT
481CGGTCTTCTT GTTTTTAAAT ATTGCACTGA TAAACATCCA TATAAATGCC AGTATCAATG
541GATACAGAAG AAACAAGACT TGCAGTTCTG ATTCAAGTAA CTTTACACGA TTTTCCAGTC
601TTATTGTATT AACCAGCACT GTGTTCATTT TCATACCCTT TACTTTGTCC CTGGCAATGT
661TTCTTCTCCT CATCTTCTCC ATGTGGAAAC ATCGCAAGAA GATGCAGCAC ACTGTCAAAA
721TATCCGGAGA CGCCAGCACC AAAGCCCACA GAGGAGTTAA AAGTGTGATC ACTTTCTTCC
781TACTCTATGC CATTTTCTCT CTGTCTTTTT TCATATCAGT TTGGACCTCT GAAAGGTTGG
841AGGAAAATCT AATTATTCTT TCCCAGGTGA TGGGAATGGC TTATCCTTCA TGTCACTCAT
901GTGTTCTGAT TCTTGGAAAC AAGAAGCTGA GACAGGCCTC TCTGTCAGTG CTACTGTGGC
961TGAGGTACAT GTTCAAAGAT GGGGAGCCCT CAGGTCACAA AGAATTTAGA GAATCATCTT
1021TGCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
1081acgaac

Download FASTA (ORF) (Full)