Construct: ORF ccsbBroadEn_08244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007943.1_s300c1, BRDN0000390724
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRBN (51185)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51185 | CRBN | cereblon | NM_001173482.1 | 98.7% | 98.6% | 683A>G;1309_1323del |
| 2 | human | 51185 | CRBN | cereblon | NM_016302.3 | 98.5% | 98.4% | 67_69delGCA;686A>G;1312_1326del |
| 3 | human | 51185 | CRBN | cereblon | XM_011533791.3 | 88.6% | 88.2% | (many diffs) |
| 4 | human | 51185 | CRBN | cereblon | XM_005265202.4 | 84.7% | 84.5% | 0_1ins186;497A>G;1123_1137del |
| 5 | human | 51185 | CRBN | cereblon | XM_024453551.1 | 64.3% | 63.1% | (many diffs) |
| 6 | human | 51185 | CRBN | cereblon | XM_011533793.2 | 60.8% | 59.1% | (many diffs) |
| 7 | human | 51185 | CRBN | cereblon | XM_011533794.2 | 60.8% | 59.1% | (many diffs) |
| 8 | human | 51185 | CRBN | cereblon | XR_940448.3 | 42.7% | (many diffs) | |
| 9 | mouse | 58799 | Crbn | cereblon | NM_021449.3 | 88.4% | 93.9% | (many diffs) |
| 10 | mouse | 58799 | Crbn | cereblon | NM_175357.3 | 88.2% | 93.7% | (many diffs) |
| 11 | mouse | 58799 | Crbn | cereblon | XR_001785159.1 | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CGGCGAAGGA GATCAGCAGG ACGCTGCGCA CAACATGGGC AACCACCTGC 121 CGCTCCTGCC TGAGAGTGAG GAAGAAGATG AAATGGAAGT TGAAGACCAG GATAGTAAAG 181 AAGCCAAAAA ACCAAACATC ATAAATTTTG ACACCAGTCT GCCGACATCA CATACATACC 241 TAGGTGCTGA TATGGAAGAA TTTCATGGCA GGACTTTGCA CGATGACGAC AGCTGTCAGG 301 TGATTCCAGT TCTTCCACAA GTGATGATGA TCCTGATTCC CGGACAGACA TTACCTCTTC 361 AGCTTTTTCA CCCTCAAGAA GTCAGTATGG TGCGGAATTT AATTCAGAAA GATAGAACCT 421 TTGCTGTTCT TGCATACAGC AATGTACAGG AAAGGGAAGC ACAGTTTGGA ACAACAGCAG 481 AGATATATGC CTATCGAGAA GAACAGGATT TTGGAATTGA GATAGTGAAA GTGAAAGCAA 541 TTGGAAGACA AAGGTTCAAA GTCCTTGAGC TAAGAACACA GTCAGATGGA ATCCAGCAAG 601 CTAAAGTGCA AATTCTTCCC GAATGTGTGT TGCCTTCAAC CATGTCTGCA GTTCAATTAG 661 AATCCCTCAA TAAGTGCCAG ATATTTCCTT CAAAACCTGT CTCAAGAGAA GACCAATGTT 721 CATATAAATG GTGGCAGAAA TACCAGAGGA GAAAGTTTCA TTGTGCAAAT CTAACTTCAT 781 GGCCTCGCTG GCTGTATTCC TTATATGATG CTGAGACCTT AATGGACAGA ATCAAGAAAC 841 AGCTACGTGA ATGGGATGAA AATCTAAAAG ATGATTCTCT TCCTTCAAAT CCAATAGATT 901 TTTCTTACAG AGTAGCTGCT TGTCTTCCTA TTGATGATGT ATTGAGAATT CAGCTCCTTA 961 AAATTGGCAG TGCTATCCAG CGACTTCGCT GTGAATTAGA CATTATGAAT AAATGTACTT 1021 CCCTTTGCTG TAAACAATGT CAAGAAACAG AAATAACAAC CAAAAATGAA ATATTCAGTT 1081 TATCCTTATG TGGGCCGATG GCAGCTTATG TGAATCCTCA TGGATATGTG CATGAGACAC 1141 TTACTGTGTA TAAGGCTTGC AACTTGAATC TGATAGGCCG GCCTTCTACA GAACACAGCT 1201 GGTTTCCTGG GTATGCCTGG ACTGTTGCCC AGTGTAAGAT CTGTGCAAGC CATATTGGAT 1261 GGAAGTTTAC GGCCACCAAA AAAGACATGT CACCTCAAAA ATTTTGGGGC TTAACGCGAT 1321 CTGCTCTGTT GCCCACGATC CCAGACACTG AAGATGAAAT AAGTCCAGAC AAAGTAATAC 1381 TTTGCTTGTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 1441 tttgttgcaa cgaac