Transcript: Human NM_001173482.1

Homo sapiens cereblon (CRBN), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-21
Taxon:
Homo sapiens (human)
Gene:
CRBN (51185)
Length:
2593
CDS:
31..1356

Additional Resources:

NCBI RefSeq record:
NM_001173482.1
NBCI Gene record:
CRBN (51185)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001173482.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000141562 CGCTGGCTGTATTCCTTATAT pLKO.1 751 CDS 100% 15.000 21.000 N CRBN n/a
2 TRCN0000141998 GCCCACGAATAGTTGTCATTT pLKO.1 1946 3UTR 100% 13.200 18.480 N CRBN n/a
3 TRCN0000141643 CCCTCAATAAGTGCCAGATAT pLKO.1 629 CDS 100% 13.200 9.240 N CRBN n/a
4 TRCN0000141985 GCTGCTTGTCTTCCTATTGAT pLKO.1 880 CDS 100% 5.625 3.938 N CRBN n/a
5 TRCN0000113342 GCTGTAAACAATGTCAAGAAA pLKO.1 992 CDS 100% 5.625 3.938 N Crbn n/a
6 TRCN0000316082 GCTGTAAACAATGTCAAGAAA pLKO_005 992 CDS 100% 5.625 3.938 N Crbn n/a
7 TRCN0000139091 CTTAACGCGATCTGCTCTGTT pLKO.1 1275 CDS 100% 4.950 3.465 N CRBN n/a
8 TRCN0000145158 GCTTGCAACTTGAATCTGATA pLKO.1 1120 CDS 100% 4.950 3.465 N CRBN n/a
9 TRCN0000144360 CAGGATAGTAAAGAAGCCAAA pLKO.1 133 CDS 100% 4.050 2.835 N CRBN n/a
10 TRCN0000142139 GCAGCTTATGTGAATCCTCAT pLKO.1 1066 CDS 100% 4.050 2.835 N CRBN n/a
11 TRCN0000141196 CCAGAAACATCTACTTGGGTA pLKO.1 1569 3UTR 100% 2.640 1.848 N CRBN n/a
12 TRCN0000113343 CAAGCCATATTGGATGGAAAT pLKO.1 1211 CDS 100% 10.800 7.560 N Crbn n/a
13 TRCN0000316081 CAAGCCATATTGGATGGAAAT pLKO_005 1211 CDS 100% 10.800 7.560 N Crbn n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001173482.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08244 pDONR223 100% 98.7% 98.6% None 683A>G;1309_1323del n/a
2 ccsbBroad304_08244 pLX_304 0% 98.7% 98.6% V5 683A>G;1309_1323del n/a
3 ccsbBroadEn_03241 pDONR223 100% 98.6% 98.6% None 66_67insGCA;1309_1323del n/a
4 ccsbBroad304_03241 pLX_304 0% 98.6% 98.6% V5 (not translated due to frame shift) 66_67insGCA;1309_1323del n/a
5 TRCN0000470663 TAACACCCAATTTACCAAAGATTT pLX_317 35.3% 98.6% 98.6% V5 (not translated due to frame shift) 66_67insGCA;1309_1323del n/a
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